FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308970
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC214516.94274525034793No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT108703.5181409198304046Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC94613.062109589927825No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA84922.7484869081140566Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC83572.7047933456322624No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT83222.693465384988834No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT77772.5170728549697383No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC76752.4840599410946047No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT72872.358481405961744No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG72182.336149140693271No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA69752.2575007282260415No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA69732.256853416189274No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC69692.2555587921157394No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC58501.8933877075444219No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA57791.8704081302391817No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA57261.8532543612648475No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC56601.8318930640515259No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA52251.6911026960546334No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC52131.687218823834029No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC51361.6622973104184873No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC50741.6422306372787001No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT49671.6075994433116483No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG49611.6056575072013464No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA48561.571673625271062No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG47681.5431918956532997No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT47021.521830598439978No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC45811.4826682202155548No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC45791.4820209081787876No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG44331.4347671294947728No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA40921.3244004272259442No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT39481.277793960578697No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG39261.2706735281742563No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA38561.2480176068874No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC37181.2033530763504547No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT37011.1978509240379325No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG31861.0311680745703466No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA31061.0052755930996538No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT29510.9551089102501862Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC28400.9191830922095997No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT28260.9146519079522284No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG27020.8745185616726542No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT26830.8683690973233648No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC25400.8220862866945011No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG24540.7942518691135061Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC22490.7279023853448555No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC19220.6220668673333981No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT18470.5977926659546234No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC18000.5825808330905913No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC17170.5557173835647474TruSeq Adapter, Index 2 (97% over 37bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT13210.4275496002848173TruSeq Adapter, Index 2 (97% over 37bp)
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA13040.4220474479722951Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA11560.3741463572515131No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC11480.3715571091044438No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC11410.3692915169757581No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT11240.3637893646632359No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT10640.3443700035602162No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA9550.30909149755639703Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT9050.29290869663721397No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT8140.2634559989643008No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC8020.2595721267436968Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC6080.1967828591772664Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG6040.19548823510373176Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT5820.1883678026992912No Hit
TGTGAAGATCCTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC5680.18383661844191992No Hit
GAGAGACAGTCGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC5540.17930543418454867No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC5340.17283231381687542Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC5020.16247532122859826No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG4440.14370327216234588No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC4380.14176133605204388No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA4380.14176133605204388No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG4370.14143768003366022No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC4250.1375538078130563Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC4200.13593552772113798Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA4110.133022623555685Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT4030.13043337540861571Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC3810.12331294300417517Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC3570.11554519856296727No Hit
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG3430.11101401430559601Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGAGTAGAAGTGGTCAGACACCCAAAAGTCCACCTGAACACTATGTTCA3400.11004304625044502No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)