FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446316
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC210654.719750132193334No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT172703.869455721954848No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC164443.6843850545353516No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA134863.0216259331953146Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA115282.582923309941835No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT109042.443112055135823No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT108882.439527151166438No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC108842.438630925174092No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC100572.253336201256509No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG97132.176260765914733No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT96402.1599046415544145No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT95132.1314494662974215No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA94552.1184541894084012No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC91912.05930327391355No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA91732.055270256947992No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC89822.012475465813459No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA87841.968112279192321No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA86931.9477231378664444No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT86321.9340556914831644Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC86291.9333835219889048No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA86101.9291264485252602No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA82731.8536194086700903No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT80761.8094802785470383No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG78511.759067566477563No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC78461.7579472839871304No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC76011.7030534419559236No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC74861.6772869446759695No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT74771.6752704361931905No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA73681.6508482779017557No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC72031.6138789557174738No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC71751.60760537377105No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG70841.5872162324451735No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA69031.5466620062915064No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC66771.4960252377239445No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG56761.2717446831393002No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC55561.2448579033689133No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG55351.2401527169090958No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT45851.027299043726866No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG41380.927145789082175No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG37870.8485019582537933Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT36440.8164618790274155No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT34670.7768038788660949Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC29370.6580539348802193No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT28900.6475232794701512TruSeq Adapter, Index 7 (97% over 38bp)
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT22610.5065917421737065No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT21290.47701628442628097No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA20910.4685021374989918Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA20560.4606601600659622TruSeq Adapter, Index 7 (97% over 37bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG19250.43130875881662323No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG15820.354457379972934No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA15590.3493040805169431Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC13570.3040446679034586No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT12730.28522392206418773No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC12140.2720045886770808No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC11950.2677475152134362No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC11180.2504951648607713No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT11080.2482545998799057No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG10750.24086073544304934No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC10430.23369092750427947No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC10400.2330187580100198No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC10090.22607300656933652Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC9500.2128536731822296No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA9450.21173339069179686No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC7740.1734197295189955Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG7240.16221690461466765Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GAGAGACAGTCGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC6800.1523584186988591No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC6770.15168624920459944Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC6510.14586078025434893Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT6380.1429480457792237No Hit
TAGGAGAGACCTTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT5200.1165093790050099No Hit
TGTGAAGATCCTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC5040.11292447503562497No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC4890.10956362756432661Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT4630.10373815861407613Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATCTCCTACCACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT4610.103290045617903No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA4600.10306598911981645No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)