FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441308
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC311847.0662666437046235No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA155873.5320003263027187No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG154373.498010459814914No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA150683.414395388254915No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT145023.286140292040933No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA131702.9843102776292296No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT131452.9786452998812623No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT131372.9768325070019124No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC120422.7287064816409403No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT108632.461546131046797Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA105302.386088627443871No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC105142.382463041685172No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG103412.343261395669238No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC99482.2542079454711903No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA99432.2530749499215967No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT98752.2376662104471254No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG91042.0629582966998106No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT87401.980476220689405No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC85971.9480725479710317No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC84211.908191104625341No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC80251.8184578570975374No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT79801.8082608971511962No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA79471.8007831265238792No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC79221.7951181487759114No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG78951.7889999728081067No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA76201.7266852175804654No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG72681.6469223308890841No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA71651.6235826225674586No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA71151.6122526670715238No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA67431.5279577981817687Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC62481.415791238772014No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG62461.4153380405521767No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCACTGC57241.2970533051746174No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT54581.2367779419362441No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT50061.1343551442529933No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC48951.109202643052018No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC33960.7695305772838924No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG27600.6254135433756016Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT24980.5660445765769032TruSeq Adapter, Index 7 (97% over 38bp)
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA23820.5397590798263344Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT21250.4815231085772295Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT20270.4593163958051973No Hit
ATCATCTTTGTCATCAGCTTAAGATGAAATAGGATGTAATCAGACGACAC20120.4559174091564169No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA19610.44436085455056334TruSeq Adapter, Index 7 (97% over 37bp)
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA17640.39972082989658014No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA17400.39428245125853145Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT15990.3623319767599953No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT15830.35870639100129614No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC13960.31633235744649996No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC12730.28846066692650035Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT11890.26942634169332985No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC10860.24608663337170414No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC10720.24291424583284238No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG10600.240195056513818No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG10150.2299980965674767No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC9270.2100573748946314No Hit
TGTGAAGATCCTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC8790.19918061761853398No Hit
GAGAGACAGTCGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC7790.17652070662666436No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC7130.16156516537203042No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT7060.15997897160259955Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC7040.15952577338276216No Hit
CAGAGTAGAAGTGGTCAGCCTCAAAAGGCTGCCACTTGCAAAGTTTCTTC6590.14932881343642082No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC6110.13845205616032338No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC5520.1250827086751203Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT5230.11851133448747814No Hit
TAGGAGAGACCTTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT5000.11329955495934811No Hit
GGCCTCTGATTCCTCACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG4890.11080696475024246No Hit
CAGAGTAGAAGTGGTCAGACACCCAAAAGTCCACCTGAACACTATGTTCA4790.10854097365105551No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)