FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351483
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC208985.945664512935192No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT150404.2790120717075935Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG112473.199870263995698No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC110403.140976946253446No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA108823.0960245587980073No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA101152.8778063234921745No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA89212.538102838544112Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA86702.466691134421864No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC82912.3588623062850833No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC81452.317324024206007No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC76162.1668188788646963No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG72362.0587055419465523No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG69151.9673782231288568No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC68821.95798943334386No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA66221.8840171501893406No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT64111.8239857973216342No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG63661.811182902160275No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA63351.8023631299380054No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC60961.7343655311921204No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA58961.6774637749194128No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC58311.658970704130783No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC56671.612311263987163No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT53411.51956140126265No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT51871.4757470489326654TruSeq Adapter, Index 7 (97% over 38bp)
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC51381.461806118645852No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT49991.4222593980363205No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA48301.3741774139858827No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC45561.2962220078922737No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT42461.208024285669577No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG40961.1653479684650465No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC40711.1582352489309584No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT39541.1249477215114245No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA38681.1004799663141602Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC38401.0925137204359812No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT37651.071175561833716No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA36621.0418711573532717TruSeq Adapter, Index 7 (97% over 37bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT35991.023947104127369No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA35401.0071610860269202No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT34520.982124313266929Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA34510.9818398044855655No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG32640.9286366623705842Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT32110.9135576969583167No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG31470.8953491349510503No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC30980.881408204664237Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG29980.8529573265278833No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC29760.8466981333378855No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA28820.819954307889713Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC21930.6239277575302362No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT15610.4441182077084809No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG14520.4131067505398554No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC14170.4031489431921316Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT13340.3795347143389581No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC10700.30442439605898436No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT10230.29105248333489814No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA9890.2813791847685379No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT9760.27768057061081186TruSeq Adapter, Index 7 (97% over 38bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT9670.27511999157854006No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC9660.27483548279717657No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT9210.2620325876358174No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC8920.2537818329762748No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT8040.22874506021628355No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC7660.21793372652446916No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC7330.20854493673947244No Hit
GAGAGACAGTCGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC7270.20683788405129125No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC6440.18322365519811767Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG6430.18293914641675416Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC6160.17525740931993866No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT5980.170136251255395Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT5860.16672214587903256TruSeq Adapter, Index 7 (97% over 38bp)
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT5810.16529960197221488Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC5760.16387705806539718No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC5400.15363474193630988No Hit
TGTGAAGATCCTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC5300.1507896541226745No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC5090.14481496971404023Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC4680.1331501096781352Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA4440.12632189892541032TruSeq Adapter, Index 7 (97% over 35bp)
TAGGAGAGACCTTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT4100.11664860035905007No Hit
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC4090.11636409157768654Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT3970.11294998620132411Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG3920.11152744229450642No Hit
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG3680.10469923154178154Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT3630.10327668763496385TruSeq Adapter, Index 7 (97% over 38bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT3570.10156963494678264No Hit
GGCCTCTGATTCCTCACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG3550.10100061738405555No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)