FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484892
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC369337.61674764689869No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA159273.28464895275649No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA158623.2712439058594494No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT157093.2396904877787223No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA153373.1629723732295028No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG147673.045420423516989No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT146983.0311904506570535No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC135582.796086551232027No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG128002.6397630812634567No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT122032.516642881301403No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA118952.4531235821585016No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA118312.439924766752184No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC117112.415176987865339No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG107962.2264751738531467No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT105502.1757422271351143No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG104312.1512006797389938No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC101352.090156158484776No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA99982.0619024442556277No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG99022.042104221146152No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC97042.001270385982858No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA95371.966829727031999No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA92031.8979484091302807No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC88191.8187555166923768No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA80011.6500581572803839No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC78061.6098430165892612No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC77551.5993252105623519No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC74601.5384869207988583No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC69851.4405269627050972No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT69701.4374334903442416No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT67871.3996931275418032Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC65301.3466916344258104No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC60351.2446070465175751No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC58511.2066604522244129No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC57841.1928429423459244No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT56391.1629393761909868No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT53521.1037509383532829No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT50551.0425001856083416No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG50261.036519472377354No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC47350.9765061085767553No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT44140.9103058000544451No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA32210.664271631621062Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT23860.4920683368667662No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG20770.4283428062331405No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT18510.38173448932958265Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA17340.3576054049149089Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT15780.3254332923620105No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC15550.3206899680753652Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT14920.30769738415977166TruSeq Adapter, Index 7 (97% over 38bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG14760.3043976803081923No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA14430.29759204111431No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT14210.2930549483183884No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG13380.2759377345883207No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA13220.27263803073674137Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG13170.27160687328312283Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT12610.26005790980259524No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC11460.23634128836936885No Hit
GAGAGACAGTCGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC11380.23469143644357918No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA9920.2045816387979179TruSeq Adapter, Index 7 (97% over 37bp)
TGTGAAGATCCTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC9580.19756976811331184No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC8860.18272110078120488No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC8570.17674038755021737No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC7670.1581795533850837No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC6230.12848221872086982No Hit
TAGGAGAGACCTTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT6120.12621367232290903No Hit
CAGAGTAGAAGTGGTCAGCCTCAAAAGGCTGCCACTTGCAAAGTTTCTTC5810.1198204961104741No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC5340.11012761604645982Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CAGAGTAGAAGTGGTCAGACACCCAAAAGTCCACCTGAACACTATGTTCA5240.10806530113922276No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCAGGTTTTGCCTCAG5020.10352820834330118No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC4870.10043473598244557No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)