FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487299
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT5555311.400187564513779Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA370527.603545256608365Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA236314.849384053732924TruSeq Adapter, Index 7 (97% over 37bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC228284.684598162524447No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC183763.770990705911566No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC142492.9240774144826895No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC135332.7771450382619296No Hit
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC131802.7047049142313035Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC127472.615847764924615No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT123652.537456469231416No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT116442.3894980289309027Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA90631.859843750961935TruSeq Adapter, Index 7 (100% over 37bp)
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC74101.5206269661952927No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG71381.4648090802566802Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG61851.2692412666555852No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC60011.2314821085206413No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT59901.2292247675451828No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC57871.1875665659071741No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA55731.1436510232937067No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA55671.142419746398002No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG53661.101171970391895Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA52841.0843445194839307TruSeq Adapter, Index 7 (97% over 37bp)
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC52601.079419411901112No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA50621.0387872743428572Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT50541.037145571815251No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT49041.0063636494226338No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG48430.9938456676496361No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC47550.9757869398459672No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT47280.9702461938152962No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC44070.9043728798950952No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA40460.830291053336863Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG38170.783297318484134No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA38030.7804243390608231No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAAGC37270.7648281650485637TruSeq Adapter, Index 7 (97% over 37bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC36630.7516945448277136No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC36270.7443068834534854No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC35430.7270690069136198Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT30340.6226156835946718No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT28450.5838304613799741No Hit
ATACCAGGGGCCATGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACC24950.5120059757972005Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC22370.4590610692818988No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA19650.4032431833432861TruSeq Adapter, Index 7 (97% over 37bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT18670.38313232738010955No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT18130.3720508353187673No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA18040.3702039199752103No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC17620.3615849817052774Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC17400.3570702997543603Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGCAAGTGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAGT16910.34701487177277196Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT15800.32423624920223515Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC15510.3182850775396625Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT14760.3028941163433539Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC14750.30268890352740313No Hit
TCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGTCTGAACTCCA14670.3010472009997968Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT13460.2762164502697523No Hit
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT11430.23455824863174357Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC10300.21136920042930518No Hit
ATACCAGGGGCCATTGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTC10180.20890664663789582No Hit
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG9460.19413132388943954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACC9350.19187398291398094Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT8750.17956121395693406No Hit
CAGAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAACTCCAG8130.16683801936798556Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC7960.16334940149682228No Hit
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG7780.15965557080970819No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA6560.13461960726371283TruSeq Adapter, Index 7 (97% over 37bp)
CAGAGTAACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT6300.1292840740489925Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT6170.12661630744163235Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCATCATTAGCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA6080.1247693920980753TruSeq Adapter, Index 7 (97% over 37bp)
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA6060.12435896646617375No Hit
ACTCAAGAAGCAGAAAGGGAAGAGCACACGTCTGAACTCCAGTCACCAGA5990.12292247675451827TruSeq Adapter, Index 7 (97% over 34bp)
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG5820.11943385888335499Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG5630.11553481538029012No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT5620.11532960256433936Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGGAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCA5530.11348268722078231Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT5340.10958364371771746No Hit
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG5150.10568460021465259Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)