FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428605
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT5758213.434747611437103Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT238365.561297698346963Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA230605.380245214124893Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA183664.285064336626964TruSeq Adapter, Index 7 (97% over 37bp)
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC142773.3310390686062923No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG136043.1740180352539054No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG127832.9824663734674117Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA90262.105901704366491TruSeq Adapter, Index 7 (100% over 37bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC75801.7685281319629962No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA73451.7136990935710035Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC73131.7062330117474132No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA70051.6343719741953546No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG66391.5489786633380385Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC63191.4743178451021337Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA63081.4717513794752743Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC62891.4673183933925176No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC61101.4255549981918083No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT52931.2349365966332637No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC49981.166108654822039No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC48381.1287782457040865Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT39110.9124951878769496No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC37280.8697985324482916No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA36160.8436672460657247TruSeq Adapter, Index 7 (97% over 37bp)
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT35430.826635246905659No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT34030.7939711389274507No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA33780.7881382625027706No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC31250.7291095530850084No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG29790.6950455547648768No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT29590.6903792536251327No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC29330.6843130621434654Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC28870.6735805695220541No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC28680.6691475834392973No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAAGC28070.6549153649630779TruSeq Adapter, Index 7 (97% over 37bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC27880.650482378880321No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA27280.636483475461089No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC27270.6362501604041017No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC26850.6264509280106392No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC26060.6080190385086501Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA24690.5760548757014033No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT24580.5734884100745441No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT23290.5433907677231951No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC20910.48786178416024073No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC18920.4414320878197875No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA17480.40783471961363027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA17340.4045683088158094TruSeq Adapter, Index 7 (97% over 37bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC17210.4015352130749758No Hit
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG16750.3908027204535645Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT16350.3814701181740764Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT15790.36840447498279305Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC15600.36397148890003617No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC14710.34320644882817514Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG14460.3373735724034951No Hit
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG14030.32734102495304535No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT12980.30284294396938904No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT12710.2965434374307346No Hit
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG12620.2944436019178498Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG12340.28791078032220807Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATACCAGGGGCCATGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACC12200.28464436952438726Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACTCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGAACTCCAGT11850.27647834252983516Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT11710.2732119317320143Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC11400.265979164965411No Hit
TGTGAAGATCTTGACCAATTGCCAAAGTCATGATCGGAAGAGCACACGTC11250.262479439110603No Hit
TCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGTCTGAACTCCA11130.25967965842675655Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CAGAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAACTCCAG10860.2533801518881021Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA10450.2438142345516268TruSeq Adapter, Index 7 (97% over 37bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC10320.24078113881079313No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT9990.2330817419302155No Hit
TGGACAGCAGGAATGTGTCAAGAAGAGATCGGAAGAGCACACGTCTGAAC9660.22538234504963778Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACC9430.2200160987389321Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT8860.20671714049066156Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC8620.20111757912296868No Hit
ATACCAGGGGCCATTGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTC8260.1927182370714294No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA8090.18875188110264696No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT8010.18688536064674935Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA7560.17638618308232523No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC7520.17545292285437641Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GAGAGAACGCGGAATTGGTCAGGAGCCACTTGGATCGGAAGAGCACACGT7490.1747529776834148No Hit
GAGAGAACGCGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACA7130.1663536356318755No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG7080.16518706034693947No Hit
CAGAGTAACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT6830.15935418392225942Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGAGTAGAAGTGGTCAGACGACACAGGGATCGGAAGAGCACACGTCTGA6600.15398793761155377Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAGAGTAGAAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAA6060.14138892453424481Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGCTGATCGGAAGAGC5900.13765588362244957No Hit
TTGGAATATGAAGCAAGAAAGGGGAGGATCGGAAGAGCACACGTCTGAAC5680.13252295236873113Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA5630.1313563770837951Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC5300.1236569802032174Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA5230.12202377480430698TruSeq Adapter, Index 7 (100% over 35bp)
TCATCATTAGCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA5020.11712415860757573TruSeq Adapter, Index 7 (97% over 37bp)
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG5000.11665752849360134No Hit
GTGTGTCACCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACACGTC4960.11572426826565252No Hit
TAGGCAAGTCGAGGCAAGGTATTGATTGTATAGTGATCGGAAGAGCACAC4780.11152459723988287No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG4780.11152459723988287No Hit
TAGGCAAGTCGAGGCAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTC4740.11059133701193406Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)