FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333127
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT6627619.895115076232187Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT131633.9513458831016397Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC128343.852584749960225No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA122993.6919853389247943TruSeq Adapter, Index 7 (97% over 37bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC113613.410411044436506No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT105993.1816694533916494No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA103103.0949157528510147Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC102983.091313523070781No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC90462.7154808826663706No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA81942.459722568269759TruSeq Adapter, Index 7 (100% over 37bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG81182.4369084463282773No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG68682.0616761775539056Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC59361.7819029979557346No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA58921.7686948220948766No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT56451.694548925785061No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT53681.6113974550246601No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC53011.5912850054183538No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG40891.2274597976147237No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC39061.1725257934661555No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC35411.062957970984039No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG35261.0584551837587468Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA34381.032038832037031No Hit
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC32870.9867107739690869Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT30120.9041596748387252No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC28860.8663362621462686Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT25900.7774812609004974No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT24240.727650415607261No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC23440.7036355504057011Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC22890.6871253305796288No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG22670.6805212426491999No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC22410.6727164114586929Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC21860.6562061916326206No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA21420.6429980157717627No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC21150.6348929987662363No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA21130.6342926271361973TruSeq Adapter, Index 7 (97% over 37bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC17630.5292275918793734No Hit
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT17550.5268261053592174Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC16100.48329916218139024No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC14230.4271644147727443No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC12620.3788344985546053No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA12420.37283078225421534TruSeq Adapter, Index 7 (97% over 37bp)
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT12000.3602229780233965No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA10310.30949157528510146Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC9600.2881783824187172No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAAGC8960.2689664902574694TruSeq Adapter, Index 7 (97% over 37bp)
ATACCAGGGGCCATTGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTC8910.2674655611823719No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT8590.257859615101748No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA8540.25635868602665046TruSeq Adapter, Index 7 (97% over 37bp)
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT8480.2545575711365335Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC8180.2455519966859486Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA7370.22123694566936933Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC7370.22123694566936933No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT7340.22033638822431084Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG6790.2038261683982385No Hit
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG6780.20352598258321902Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT6700.20112449606306304No Hit
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG6540.1963215230227511Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA6510.1954209655776926No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC6440.1933196648725561No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC6400.19211892161247812No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA6050.18161241808679573No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT5950.17861055993660077No Hit
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG5790.17380758689628878No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA5110.15339495147496301No Hit
CACACCCCCAGGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTG4820.1446895628393976Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATACCAGGGGCCATGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4560.13688473164889065Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC4530.13598417420383216No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC4490.13478343094375417Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG4250.12757897138328625Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA4220.12667841393822776TruSeq Adapter, Index 7 (100% over 35bp)
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC3910.11737265367262335No Hit
ACTCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGAACTCCAGT3910.11737265367262335Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3740.11226949481729191Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGTGAAGATCTTGACCAATTGCCAAAGTCATGATCGGAAGAGCACACGTC3710.11136893737223341No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT3600.10806689340701894Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGAGTAGAAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAA3550.10656596433192146Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT3540.10626577851690197No Hit
GCTCTGATAGGAAAATGGATCCATTTTGTGGATGGGATCGGAAGAGCACA3460.10386429199674599No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG3420.102663548736668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)