FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353889
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT4716913.328755626764325Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA208975.904958899541947Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC139633.9455874582142987No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA101302.8624794780284213TruSeq Adapter, Index 7 (97% over 37bp)
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT95942.711019556979731Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC89032.5157605915979304No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT83232.351867393448228No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT81222.295069923054969No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC77652.194190833849032No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC72322.0435786362390465No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG70101.9808471017748501No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA69841.9735001653060704TruSeq Adapter, Index 7 (100% over 37bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC68511.9359177595234665No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT68431.9336571636869186No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT61241.7304861128772016No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG61151.7279429425610855No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG60691.7149445165009367Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC60501.7095756013891361No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC57231.6171737465702525No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA55771.575917872553258Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC49971.4120246744035558No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA47011.328382628451294Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA44461.2563261361613387No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAAGC41181.1636417068628864TruSeq Adapter, Index 7 (97% over 37bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT39101.1048662151126483No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC39091.1045836406330798No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC37111.0486338936785264No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA35841.0127469347733329No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT34360.9709259117972019No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC33220.9387124211263984No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG32210.9101723986899848Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC31120.8793717804170234No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC28820.8143796501162793Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC27370.7734063505788538Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC26990.7626685203552527No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG26990.7626685203552527No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA25130.7101096671555205No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC24180.6832650915965175No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA19230.5433907242101337No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC19010.5371740856596278Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA17190.4857455303781694TruSeq Adapter, Index 7 (97% over 37bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC17000.4803766152663688No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT15140.42781776206663674No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT14500.4097329953742557No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC14450.40832012297641357Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT14350.405494378180729No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC14160.4001254630689284No Hit
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT12550.3546309718584076Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC12490.3529355249809969Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC11640.32891669421767844No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA11130.31450539575968733TruSeq Adapter, Index 7 (97% over 37bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT10810.3054630124134969No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT10610.2998115228221278Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT10500.29670320354687485No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG10440.29500775666946416No Hit
TCATCATTAGTGGATCTACAGATCGGACATTCAAAGTGTGGAATGCAGAG10340.2921820118737796No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC10160.28709567124154745No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC9760.2757926920588094No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA9490.26816318111046117No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA9230.26081624464168146TruSeq Adapter, Index 7 (97% over 37bp)
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA7870.22238611542037193No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT7350.20769224248281243No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA7300.20627937008497016No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT6870.1941286674635267Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT6790.19186807162697908No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC5930.16756666638409218No Hit
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG5620.15880685751747017Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC5480.15485081480351184No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA5420.15315536792610113No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA5240.14806902729386898No Hit
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT4880.1378963460294047Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG4580.12941911164235112Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG4400.12433277101011898No Hit
ATACCAGGGGCCATTGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTC4360.12320247309184518No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT4160.11755098350047614No Hit
ATACCAGGGGCCATGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4100.11585553662306543Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC3860.10907374911342257Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG3850.10879117463385411No Hit
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG3710.1048351319198958Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA3700.10455255744032732No Hit
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG3690.10426998296075889No Hit
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3670.10370483400162199Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GAGAGAACGCGGAATTGGTCAGGAGCCACTTGGATCGGAAGAGCACACGT3590.10144423816507435No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)