FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318950
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC192866.046715786173381No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC159505.000783821915661No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC140334.3997491769869885No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT127984.012541150650572No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC119083.7335005486753414No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC118963.7297382034801694No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT105373.3036526101269796Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT100433.1487693995924126No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA99103.1070700736792602Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG86022.6969744474055495No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC70032.195641950148926No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG64462.0210064273397084Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT63852.0018811725975856Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT63581.9934158959084496No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT62821.9695877096723622No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC60941.910644301614673No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC57481.802163348487224No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG53331.6720489104875373No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA48951.5347233108637717No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA48721.5275121492396928TruSeq Adapter, Index 7 (97% over 37bp)
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG48401.517479228719235No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC44911.4080576892929928No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA39621.2422009719391753No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT37891.1879604953754506No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA35641.117416522965982No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA32421.0164602602288761TruSeq Adapter, Index 7 (100% over 37bp)
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC31961.002037936980718No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT31310.9816585671735382No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT29040.910487537231541No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA28970.9082928358676908No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA27670.8675340962533312No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC27340.8571876469666092No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC25510.7998118827402414No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT25200.7900924909860481No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC24990.7835083868944976No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC24840.778805455400533No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG23150.7258190939018655No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA22010.6900768145477348No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT21840.6847468255212417No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC19070.5978993572660292Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA18830.5903746668756858No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC15610.4894184041385797No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA15610.4894184041385797Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT15470.4850290014108794No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC15300.4796990123843862No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC14870.4662172754350212No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA14300.44834613575795584Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA13600.42639912211945447No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG13570.4254585358206615No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG12740.3994356482207242Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA12660.39692741809060983No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA12200.3825050948424518No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC11760.3687098291268224No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT11580.3630663113340649No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC11120.3486439880859069No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC9610.30130114437999683Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC9390.29440351152218214No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC9070.2843705910017244No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA8240.25834770340178714No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC8070.25301771437529397No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA7910.24800125411506505TruSeq Adapter, Index 7 (97% over 37bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC7250.22730835554162096No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT6990.21915660761874903No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA6960.2182160213199561TruSeq Adapter, Index 7 (97% over 37bp)
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA6940.2175889637874275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAAGC6830.21414014735852013TruSeq Adapter, Index 7 (97% over 37bp)
TCATCATTAGTGGATCTACAGATCGGACATTCAAAGTGTGGAATGCAGAG6780.21257250352719861No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG6390.20034488164289072No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT6340.1987772378115692No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT6070.19031196112243298Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC5560.17432199404295345Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG5450.1708731776140461Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC5380.16867847625019597Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA4880.15300203793698072No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC4530.14202853111773006Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG4470.14014735852014423Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT4400.1379526571562941No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAA4120.12917385170089357TruSeq Adapter, Index 7 (97% over 37bp)
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC3980.12478444897319328Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG3910.12258974760934314No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG3370.1056591942310707No Hit
GTGTGTCACCTTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC3350.10503213669854208No Hit
TGTCTCAGTGCTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC3340.10471860793227777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)