FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435330
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT5132811.790595640089128Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA197094.527370041118233Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG167273.84237245308157No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC144493.3190912640984997No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT115702.6577538878551903No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT112882.5929754439161097No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG106562.4477982220384535TruSeq Adapter, Index 3 (97% over 36bp)
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC104612.4030046171869617No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT101292.32674063354237No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC97722.2447338800450236No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC88412.0308731307284127No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT87672.013874531964257No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG86101.9778099372889533No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT81431.8705349964394828TruSeq Adapter, Index 3 (97% over 36bp)
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG79751.8319435830289665Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC67191.5434268256265362No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT66331.5236716973330577Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC63811.4657845772172833No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC60261.384237245308157No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA60241.3837778237199365Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC56111.2889072657524177Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA50491.1598097994624768No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG47321.0869914777295386No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT46641.0713711437300437No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA44371.0192267934670252Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT43050.9889049686444766No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC41750.9590425654101487No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT40910.9397468587048905No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA39900.9165460684997588No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA36300.8338501826200813No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC36210.8317827854730894Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC35780.8219052213263501No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC33470.7688420278868904No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC32900.7557485126226082No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC31150.7155491236533205No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA30080.6909700686835275No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT29680.6817816369191187No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG27580.6335423701559736No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA26270.6034502561275354No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT25880.5944915351572371No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA25090.57634438242253No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT24610.5653182643052398No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT24260.5572783865113822No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC22390.5143224680127719No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC22010.5055934578365837No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA21230.48767601589598697No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA21170.4862977511313257No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC20510.4711368387200514No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA19590.45000344566191164No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG19220.4415041462798337Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC19160.4401258815151724No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA18750.43070773895665354No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC17420.40015620333999496No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA16900.3882112420462637No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG16300.37442859439965087TruSeq Adapter, Index 3 (97% over 36bp)
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA16020.3679966921645648No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG15630.3590379711942664No Hit
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT14520.3335400730480325Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC14380.3303241219304895No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC13790.3167711850779868No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT13690.31447407713688463No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC13450.3089610180782395No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT13390.3075827533135782Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC12990.29839432154916956No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC12690.29150299772586313No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG12410.28507109549077714TruSeq Adapter, Index 3 (97% over 36bp)
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG11690.2685319183148416No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT11640.26738336434429055No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC11200.25727608940344104Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC10910.25061447637424483Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CACACCCCCAGGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTG10100.2320079020513174Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG9820.22557599981623136No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC9030.20742884708152437Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC7960.18284979211173133No Hit
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA7560.1736613603473227TruSeq Adapter, Index 7 (97% over 35bp)
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG7120.16355408540647326TruSeq Adapter, Index 3 (97% over 36bp)
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGCTGATCGGAAGAGC7000.16079755587715067No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC6940.15941929111248937No Hit
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG6940.15941929111248937Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG6920.15895986952426894Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCTCTGATAGGAAAATGGATCCATTTTGTGGATGGGATCGGAAGAGCACA6900.15850044793604853No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG6880.15804102634782807No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG6380.14655548664231732No Hit
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC6340.14563664346587646Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG5990.13759676567201895No Hit
GTGTGTCACCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACACGTC5890.13529965773091676No Hit
GTGTGTCACCCTCACCAAAACTTTCTCGATCGGAAGAGCACACGTCTGAA5800.13323226058392484Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT5560.12771920152527969No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA5480.12588151517239796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG4790.1100314703787931No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT4750.10911262720235224Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTACTGAAGTGGGATGCCATTGTCACAGAGGATAAGGATCGGAAGAGCAC4730.1086532056141318No Hit
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA4680.10750465164358074Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4460.10245101417315601Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA4380.10061332782027427No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)