FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340175
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC4283412.591754244139047No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC3436010.100683471742485No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC285318.387153670904683No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC270287.94532226060116No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC185535.453957521863747No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT172125.05974865877857No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT159214.680238112736092No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT152294.476813404865143No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG151294.447416770779745No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT114743.3729697949584776No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC90932.6730359373851695No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT86092.5307562284118466Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT85322.508120820166091No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG44141.2975674285294334No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA43411.2761078856470935Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG40861.2011464687293305Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG38971.1455868303079297TruSeq Adapter, Index 3 (97% over 36bp)
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT28260.8307488792533254No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT26400.7760711398544866Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT23970.7046373190269714No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT22330.65642683912692TruSeq Adapter, Index 3 (97% over 36bp)
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG20810.6117439553171162No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC19710.5794076578231793No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT18670.5488351583743661No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC17470.5135591974718894No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA16270.47828323656941274No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA16190.47593150584258104Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA16140.47446167413831114No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT13440.39509076210773864No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC12210.3589329021827001No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC11540.33923715734548393No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT11330.3330638641875505No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC10990.3230690085985155Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA10850.31895347982655986Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GTGTGTCACCTTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC9030.2654516057911369No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC7150.21018593371059016No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG7140.20989196736973617TruSeq Adapter, Index 3 (97% over 36bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC6500.19107812155508194No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA6230.1831410303520247No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA6010.1766737708532373No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC5960.17520393914896742No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG5820.17108841037701183TruSeq Adapter, Index 3 (97% over 36bp)
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC5790.17020651135444992No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT4810.14139780995076062Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGTCTCAGTGCTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC4570.13434261777026532No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA4490.13199088704343354No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA4390.1290512236348938No Hit
ATCCAGACTGCGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT4390.1290512236348938No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG4140.12170206511354449Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCATCATTAGCGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG4020.11817446902329684No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC4010.11788050268244285Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT3930.11552877195561108No Hit
CATCTCCTACCACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT3890.1143529065921952No Hit
GTGTAGAAATGGCCGGGTTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC3510.10318218563974425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)