FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335329
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT4311512.857522015692052Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC201766.016777552791438No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG195095.81786842175893No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC159754.7639780633348145No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT141394.216456077464222No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC115363.440203501635707No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG115093.432151707725845TruSeq Adapter, Index 3 (97% over 36bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG96102.8658422027322423No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT88302.6332348231140164No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA86892.5911865660291835Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT86032.565540111353328No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC81222.4220988939220884No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT80112.3889970745148794TruSeq Adapter, Index 3 (97% over 36bp)
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG80012.386014928622338Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC77512.3114612813088042No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT72462.160862913735466No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC64961.9372019717948643No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA61241.8262661445923256Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT57141.7039981629981302No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT54481.62467308225653Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA47711.4227818053314805Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA47211.4078710758687738No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC34821.0383831997828998No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC27580.8224758371629056Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA22330.6659131778044846No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT21780.6495113753955072No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC20830.6211809894163642No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT19680.5868863116521387No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG18030.5376809044252062TruSeq Adapter, Index 3 (97% over 36bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT16960.5057719433750137No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC16420.4896683555552905No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG14550.4339022273647671TruSeq Adapter, Index 3 (97% over 36bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG14050.41899149790206036Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC13170.39274861404769645No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT13060.3894682535659009Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA13040.3888718243873927No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC13000.3876789660303761No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT12430.3706807344428904No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC11820.35248964449838815No Hit
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT11620.3465253527133054Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC11390.3396664171604603Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT11120.33161462325059865No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG11110.3313164086613445No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG9920.29582887254010237TruSeq Adapter, Index 3 (97% over 36bp)
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT9680.28867172239800315No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC9300.277339568006346No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA8870.26451634066841817No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA8530.2543770446337776No Hit
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG7740.23081809208270088Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA7430.2215734398158227No Hit
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7340.21888950851253544Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT7140.21292521672745274Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC7000.20875021247789483No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC6910.20606628117460762No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA6900.20576806658535346No Hit
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACT6580.19622519972922114Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC5900.17594660765993994Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC5720.17057874505336548No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC5430.16193052196499558No Hit
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG5290.15775551771543767No Hit
TCATCATTAGCGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG4890.14582693414527226No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA4770.14224835907422265No Hit
CACACCCCCAGGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTG4660.13896799859242714Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC4210.12554834207599103Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA4000.1192858357016542TruSeq Adapter, Index 7 (97% over 35bp)
GCTCTGATAGGAAAATGGATCCATTTTGTGGATGGGATCGGAAGAGCACA3930.11719833357687526No Hit
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGCTGATCGGAAGAGC3910.11660190439836697No Hit
ATCCAGACTGCGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT3850.11481261686284218No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC3820.11391797309507977Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG3520.1049715354174557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)