FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881710
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC633767.187850880675052No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC465745.282235655714464No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT465065.274523369361808No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG436254.947771943155913No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT403864.580417597622801Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC343863.8999217429767157No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT324803.683750893150809No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA305053.459754340996473Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT234042.6543874970228307No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT219562.4901611641015755No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC214832.436515407560309No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT207802.3567839765909424No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC194642.207528552471901No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC190732.163182905944131No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA155381.7622574315818127No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC143361.6259314287010467No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC139221.5789772147304668No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC137981.564913633734448No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC134681.5274863617289132No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT119641.3569087341642945No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA110481.253019700354992No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG106901.2124167810277757No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT102121.1582039446076375No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA100591.1408513003141623No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC100321.1377890689682548No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG100251.1369951571378343No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG99661.130303614567148Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA91841.041612321511608No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT91351.036054938698665No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC77110.8745505891959943No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT76230.8645699833278515No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA73140.8295244468135782No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG70820.8032119404339295No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC68290.7745176985630196No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA65770.745936872667884No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA65400.7417404815642331No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC64880.735842850823967No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA61210.6942191877147815No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC59600.6759592156151115No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT57950.6572455796123442No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG57200.6487393814292681TruSeq Adapter, Index 3 (97% over 36bp)
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA52570.5962277846457452No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG43840.49721563779473976No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA39750.4508285037030316No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT39220.44481745698699116TruSeq Adapter, Index 3 (97% over 36bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG38630.4381259144163047No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA36930.4188451985346656Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG31910.3619103786959431No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT28460.3227818670537932No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA26650.3022535754386363Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC24040.2726520057615316No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT23730.2691361105125268No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT22720.2576810969593177No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC22540.25563960939537944No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC18890.21424277823774257No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT17130.1942815665014574No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC16150.18316680087557133No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC15570.17658867428065916No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC15390.1745471867167209Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC13760.15606038266550226No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA13330.15118349570720532No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTT13110.14868834424016966No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT12920.14653344070045707No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGC12470.14142972179061142No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC12080.13700649873541187No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT11750.1332637715348584Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA11320.12838688457656144Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC10800.12248925383629539Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG10800.12248925383629539Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG10700.1213550940785519TruSeq Adapter, Index 3 (97% over 36bp)
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGCTGATCGGAAGAGC9890.11216840004082974No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACT9630.10921958467069671No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC9510.10785859296140454No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC9310.10559027344591758Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)