FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002318850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019352
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT574815.638974564232964Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT547195.368018113468164No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG427164.190505340647785No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC369863.628383522080694No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT339043.3260345788304724No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC288302.8282673698584984No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT281422.760773511014841No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT274242.6903366060006753No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC270132.6500168734647107No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA260862.5590767467960034Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC233422.2898861237335093No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC228032.237009394203376No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC217512.133806575157551No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG203081.992246054356101No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC202611.9876352820223042No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG197051.9330908263288835Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC180361.7693593577096038No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA171921.6865616587793029No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC149501.4666180083033142No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT147401.446016685109756No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT141351.3866652540045048No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT131361.2886618165265777No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG128151.2571712225021385TruSeq Adapter, Index 3 (97% over 36bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA108891.0682276583555042No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA108271.0621453629364537No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG102311.003676845682355No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA100820.9890597163688304No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA97740.9588444423516116No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA96360.9453064299672733No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC94880.9307874021927657No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT93830.9204867405959865No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT91710.8996892143243944No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA91250.8951765435296148No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT89400.8770277588114802TruSeq Adapter, Index 3 (97% over 36bp)
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG88770.8708473618534128No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC87380.8572112479300574No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC86120.8448504540139226No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC81230.7968788014346369No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA79530.7802015398017564No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC75650.7421381426631821No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT73670.7227140379378272No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA73110.7172203517528783No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG70330.6899481239061678No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG70170.6883784992818968No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA69030.6771949238339651No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA64990.6375619020711196Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC63900.6268688343182728No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC61300.6013624341738674No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT56440.5536850862116325No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG54190.53161223993282No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT52530.5153273844560073No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT51610.5063020428664484Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA50000.49050769508472053Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC45290.4443018702077398No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC41360.4057479653740808No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC39240.3849504391024886Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC32230.3161812602516108No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA31690.31088377714469584No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA31250.3065673094279503No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC28420.2788045738861551No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC25680.25192475219551247No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA25110.24633296447154662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC24290.2382886382721572No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC22400.2197474473979548No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG20600.20208917037490484No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG19610.19237711801222737Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG18310.17962391794002464Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT17450.17118718558456744No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT16330.16019981321466972Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG16220.15912069628548334TruSeq Adapter, Index 3 (97% over 36bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC16200.15892449320744945Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC15080.1479371208375517No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG14930.14646559775229753No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGC14500.14224723157456895No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC13790.1352820223043659Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAGAGTAGAAGTGGTCAGACACCCAAAAGTCCACCTGAACACTATGTTCA13210.12959213304138315No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG13160.12910162534629843TruSeq Adapter, Index 3 (97% over 36bp)
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGCTGATCGGAAGAGC13080.1283168130341629No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT11600.11379778525965516No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA11480.11262056679145183No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC10520.10320281904582519Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA10430.10231990519467268No Hit
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG10300.10104458518745242Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)