FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581366
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC412917.102410529683538No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC388976.690621742585566No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT364466.269028460556688Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT334385.751626342097749No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC320345.510126151168111No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC198583.4157484269805938No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC198353.4117922272716323No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC163022.804085550238576No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG126572.177113900709708No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT118242.033830667772109No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT108171.8606179239928033Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCT101181.7403838545769792TruSeq Adapter, Index 7 (97% over 36bp)
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA100691.7319554291100614No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC98021.6860291107495107No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT95841.6485312178558773No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC94921.6327064190200322No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT93301.6048410123743047No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT91941.5814478314865335No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC89591.5410257909819287No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC88321.5191806882411423No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG84171.4477970847968404No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA82121.4125353047821854Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC81911.4089231224392207No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA79471.3669530037876312No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT63181.0867508591833714No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC63111.0855467984023834No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGC59611.0253437593529722TruSeq Adapter, Index 7 (97% over 36bp)
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA54050.9297069316059074Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC52520.9033896031071649No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG45100.7757591603224131Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA45060.7750711255904198Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC44490.7652666306595157No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC40170.6909588796042424No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT37880.6515688911976276No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA27200.4678636177554243No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG25830.44429842818465476No Hit
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC24530.42193729939487346Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG24000.4128208391959626Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA23910.41127276104897775No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG23500.4042204050460467No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA21390.3679265729334017No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC21150.3637983645414421No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA18840.32406435876883066No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG18790.3232043153538391No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT18610.32010815905986933No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA18370.31597995066790974No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCT17220.29619895212310315TruSeq Adapter, Index 7 (97% over 36bp)
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC16760.28828655270518055No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT16360.28140620538524785No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT16100.27693397962729155No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC15490.26644144996439423Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC15390.264721363134411No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG15030.2585290505464716No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG13940.23978010409965497No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC13190.22687945287478115No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT12210.210022601940946No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC11830.2034862719870099No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC11760.20228221120602166No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC11620.19987408964404524No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT11340.19505784652009234No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA11300.19436981178809906No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT11230.19316575100711084No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG11160.1919616902261226No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA10990.1890375426151512No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC10950.18834950788315796Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT10780.18542536027218653No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTAT10140.17441680456029418TruSeq Adapter, Index 7 (97% over 36bp)
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACT9860.1696005614363413No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC9610.16530034436138338No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA9560.16444030094639178No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC9530.16392427489739683Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG9530.16392427489739683Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCT9350.1608281186034271TruSeq Adapter, Index 7 (97% over 36bp)
CAGAGTAGAAGTGGTCAGCCTCAAAAGGCTGCCACTTGCAAAGTTTCTTC9290.15979606650543718No Hit
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT9190.158075979675454Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTT8820.15171165840451628No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA8260.14207917215661045No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT8200.14104712005862055No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCC8140.14001506796063065No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT6950.11954603468383085Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA6520.11214966131490318No Hit
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC6450.11094560053391495Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC6260.10767743555694692No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)