FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599234
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC305435.097007179165401No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC291814.869717005376865No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT267294.460527940670923Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT254434.2459206253316735No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC246244.109246137568963No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC245084.089888090462156No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC167842.800909160695154No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC163602.7301521609254484No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA155812.6001528618202574Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT150492.511372852675249No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC150202.5065333408985473No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT149842.5005256711067796No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG139762.3323109169372898No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC135852.2670609478100374No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT130902.1844554881732345No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG122622.046279082962582No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA120102.0042253944202097No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT116581.9454837342340388Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC104321.740889201881068No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG99401.6587843813935792No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT97061.6197345277470905No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC96311.6072185490142417No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT94071.5698374925321328No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA89751.4977454550309228No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA86431.4423413891735115No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC82431.3755895025983171No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT75771.2644476114506187No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC71271.1893517390535249No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT69671.1626509844234472No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA66071.1025742865057724No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG59410.9914323953580738No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA59090.9860922444320583No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC57510.9597252492348565No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC55440.9251811479321935No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT47230.788172900736607No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCT37540.6264664555081988TruSeq Adapter, Index 7 (97% over 36bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC33500.5590470500672525Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA33100.552371861409733No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA31750.529843099690605Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGC30930.5161589629426901TruSeq Adapter, Index 7 (97% over 36bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC27860.4649268899962285No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA27000.45057523438256175No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA25180.4202031259908483Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG24430.40768714725799937Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG22950.3829889492251775Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC21340.3561213148786618No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG21310.3556206757293478No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT18580.31006251314177763No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC18260.30472236221576215No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA16690.2785222467349983No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC16410.27384961467473473Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT15270.25482532700080435No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA15160.25298965011998653No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG14580.24331062656658334No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC14100.23530040017756002Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC13100.21861242853376145Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC12280.2049282917858466No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT11370.18974223758998987No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT10540.17589122112563704No Hit
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC9570.15970388863115245Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC9520.1588694900489625No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT8410.14034584152434607No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGT7850.13100057740381887No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT7790.12999929910519095No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC7640.12749610335862116No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC7270.1213215538504157No Hit
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG7060.117817079805218Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC6820.11381196661070633Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGCAAGTCGAGGCAAGGTATTGATTGTATAGTGATCGGAAGAGCACAC6790.11331132746139239No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCC6550.10930621426688072No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGT6460.10780429681893884No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC6370.10630237937099697No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTAT6280.10480046192305509TruSeq Adapter, Index 7 (97% over 36bp)
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGT6180.10313166475867525No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT6120.10213038646004734Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)