FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884974
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT563296.365045752756577No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC551306.229561546440912No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC445665.035854160687206No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC442424.99924291561108No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC387994.384196597866152No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC335623.792427800138761No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT318653.600670754169049No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG306913.4680114896030845No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT265983.005512026342017No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT194792.2010816136971254No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT187832.1224352353854465No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA184012.079270125450013No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC183642.0750892116604556No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC161181.8212964448673068No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA158871.7951939831000685No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC157001.774063418812304No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC153981.7399381224759143No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA150221.6974509985604098No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT149411.688298187291378No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA149141.6852472502017009No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA139881.5806114077927713No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG137991.5592548481650308No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC136531.5427571883467763No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT133861.5125868104599685No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG133551.509083882690339No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC132571.4980101110315105No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC127741.443432236427285No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT123641.3971031917321863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC121941.377893587834219No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT116581.317326836720627No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA95211.0758508159561748No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA90801.0260188434914472No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA90311.020481957662033No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA84400.9537003346990985No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC79270.8957325299952315No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG72120.8149391959537794No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG71280.8054473916747837No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA62720.7087213861650172Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT60620.6849918754675279No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG54990.6213741872642586No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG54080.6110913992953465No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT32430.36645144377122946No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCT27300.3084836390673624TruSeq Adapter, Index 7 (97% over 36bp)
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG27280.30825764372738634Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC25390.2869010840996459No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGC23950.2706294196213674TruSeq Adapter, Index 7 (97% over 36bp)
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT18990.21458257530729716Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT18020.20362180131845684No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT17110.19333901334954473No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC16650.18814112053009469No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG16450.18588116713033376No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA15970.18045727897090763No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA15570.17593737217138583Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT15310.17299943275169666No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCC13940.1575187519633345No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT13390.15130388011399204No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTT12020.13582319932562992No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG12000.1355972039856538No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGT11860.13401523660582118No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA11670.13186828087604832Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGT11110.12554041135671784No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC10950.1237324486369091No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC10030.11333666299800899No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC9840.11118970726823614No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC9700.1096077398884035No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)