FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922821
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC532345.768616015456953No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT352653.821434492713105No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC286553.105152570216759No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT275242.9825935907396994No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG271192.9387064230224498No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA256502.779520622092475No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA234292.53884556159862No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT229692.4889984081419905No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA225632.4450028770476617No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG219492.37846776352077No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT219302.3764088593562565No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC217712.3591790824005954No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC214292.3221188074393626No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG213492.3134497372729923No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA212272.3002294052692775No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC210262.278448366476272No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA206652.2393291873505263No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC203332.2033525461600894No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA196902.1336748946978883No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG196792.1324828975500125No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA193142.092930264915948No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC179401.9440389848085382No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC175771.904703078928633No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT168221.8228887292335134No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT165201.7901629893554656No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC164591.7835528233536082No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT158511.7176678900891937No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC153791.666520376107609No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG152041.6475567851186743No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG151901.6460396978395593No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT149051.6151561353718653No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA142701.546345390926301No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC141051.5284654337081622No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC138871.5048422175048033No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC134091.4530445232607407No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA125301.3577931148077471No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT105801.1464845295024713No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC99541.0786490554506236No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT87740.9507802704966619Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC70090.7595189099511174No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA58520.634142482669987Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA43260.4687799692464736No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA41780.4527421894386885No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT39670.42987751687488696No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG38050.4123226497879871No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT33440.36236713295427825No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG31700.3435119053424229Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG27630.299408010871014No Hit
ATCATCTTTGTCATCAGCTGAAGATTAAATAGGATGTAATCAGACGACAC20810.22550418770270722No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC20790.22528746094854796Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG20620.2234452835381943No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC15520.1681799612275837No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGT14430.15636835312590414No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC12960.14043893669519875No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT11270.12212552596874152No Hit
GGCCTCTGATTCCTCACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG9800.10619610953803608No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA9290.10066957730697502Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)