FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771390
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT768809.966424247138281Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC389365.047511634841002No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG334464.335809383061746No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC299693.8850646236015502No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC281183.6451081813349924No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA271513.519750061577153Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC235993.0592825937593178No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC228452.9615369657371757No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC221822.875588223855637Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC194292.5187000090745277TruSeq Adapter, Index 6 (97% over 36bp)
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT186212.4139540310348853No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC182602.3671553948067774No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC137871.7872930683571215No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT119701.5517442538793607No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC111561.4462204591711068No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA109521.4197746924383257No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG105571.3685684284214212Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG101741.318917797741739No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG98051.2710820726221495TruSeq Adapter, Index 6 (97% over 36bp)
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT88851.1518168501017643No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG87071.1287416222662985No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT87001.1278341694862521No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC86871.1261489000375946No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA86681.1236858139203256No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT79591.0317738109127679No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC79241.0272365470125358No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA76570.992623705259337No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC73200.9489363357056743No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC69080.8955262577943712No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA58130.7535747157728256Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT53340.6914790183953642No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA48200.6248460571176706No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG47950.6216051543317906No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA47080.6103268126369281No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA46790.6065673654053073Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA44400.5755843347722942No Hit
GCTGTGATGACTTGCCATTGATCGGAAGAGCACACGTCTGAACTCCAGTC40280.5221742568609913Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA40190.5210075318580744No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC38050.4932654040109413No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT37680.48846886788783883No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC36910.4784868873073283TruSeq Adapter, Index 6 (97% over 36bp)
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT36740.4762830734129299No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC36400.4718754456241331No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT35590.46137492059788177No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT35330.4580043817005665No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT34520.4475038566743152Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA33620.43583660664514706No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC33220.4306511621877391TruSeq Adapter, Index 6 (97% over 36bp)
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA30970.40148303711481875No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC29250.37918562594796407No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG29070.37685217594213044No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA26220.33990588418309803No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT24110.3125526646702706No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA24090.3122933924474001No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA23710.3073672202128625No Hit
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG23390.30321886464693604No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC23030.2985519646352688No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC22390.2902552535034159No Hit
GTGATGACTTGCCATTGATCGGAAGAGCACACGTCTGAACTCCAGTCACT22230.2881810757204527Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC19900.25797586175605075No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC19780.25642022841882833No Hit
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC19280.24993842284706827TruSeq Adapter, Index 5 (97% over 35bp)
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC19040.24682715617262344TruSeq Adapter, Index 6 (97% over 36bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC17870.2316597311347049No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT17170.22258520333424078Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT16170.20962159219072066No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC16060.20819559496493342No Hit
CACACCCCCAGGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTG15580.20197306161604378Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT15290.19821361438442292No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG13770.1785089254462723Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG12640.16386004485409456No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC12550.16269331985117774Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC11820.15322988371640803Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GTGTAGAAATGGCCGGATTGAAGAGCACACGTCTGAACTCCAGTCACTCC11510.1492111642619168TruSeq Adapter, Index 6 (96% over 31bp)
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG11370.147396258701824Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC11230.14558135314173118No Hit
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10980.1423404503558511Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT10980.1423404503558511Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA10750.13935881979284148No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC10520.13637718922983186Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG10360.13430301144686863No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA10270.1331362864439518Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCTGATAGGAAAATGGATCCATTTTGTGGATGGGATCGGAAGAGCACA10190.1320991975524702No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT9960.12911756698946059No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTTAAGGTGATGATCGGAAGAGCAC9690.12561739198071015No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC9480.12289503364057092Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGT8450.10954251416274516No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG8200.10630161137686514No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG8200.10630161137686514No Hit
GTGTGTCACCCTCACCAAAACTTTCTCGATCGGAAGAGCACACGTCTGAA8100.10500525026251313Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC7780.1008568946965867No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTT7740.10033835025084588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)