FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457099
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT5616112.286397476257878Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC204044.463803246123925No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC193714.237812815166955Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA177793.889529401726978TruSeq Adapter, Index 13 (97% over 38bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC158123.4592068676588665No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC138773.035885005217688No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC121142.6501917527712813No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG119262.6090628069630433No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC114502.5049278165123967No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT113402.4808630077948104Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC113232.4771439009930014Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC96262.105889533777147No Hit
TGTGAAAGCATTGATGGAATGATCGGAAGAGCACACGTCTGAACTCCAGT66001.4438885230551806Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA61261.3401910745812178Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA60631.3264085023156909No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT54901.2010527259959003No Hit
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG54501.1923018864622323Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA53301.1660493678612291TruSeq Adapter, Index 13 (97% over 38bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC50061.0951675676385204Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT49771.0888232089766112No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC49281.0781034305478683No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT43690.9558104480648613Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC42960.9398401659159176No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG42440.9284640745221494Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACCAGGGGCCATTGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTC35720.7814499703565311No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG33590.7348517498397502Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACTCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGAACTCCAGT32340.7075053762970385Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC32180.7040050404835714No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT28720.6283102785173453No Hit
TCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGTCTGAACTCCA25690.5620226690498119Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC23790.5204561812648901No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA23230.5082050059177552TruSeq Adapter, Index 13 (97% over 38bp)
GCCATTCAAACCAGGAAGATACCGGATCGGAAGAGCACACGTCTGAACTC22090.4832651132468021Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA20970.4587627625525324No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC20840.45591873970409036Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA19680.4305413050564539No Hit
TTGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACACGTCTGAA19180.4196027556393691Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGCCGATCGGAAGA19140.4187276716860024No Hit
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACA18680.40866420622228444Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT18660.40822666424560106No Hit
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA18150.3970693438401746Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAGAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAACTCCAG18110.3961942598868079Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC17430.3813178326795727No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC17230.3769424129127388No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA16640.3640349246005789Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA15950.348939726405002Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC15930.3485021844283186Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC15100.33034419239595797No Hit
TAGGCAAGTCGAGGCAAGGTATTGATTGTATAGTGATCGGAAGAGCACAC15070.32968787943093286No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC15000.32815648251254104No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC14570.3187493300138482TruSeq Adapter, Index 13 (97% over 37bp)
ATACCAGGGGCCATGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACA13470.2946845212962618Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TAGGCAAGTCGAGGCAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTC13430.2938094373428951Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGCAAGTCGAGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGT13270.29030910152942796No Hit
CATCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGCACACGTCTGAAC12470.2728074224620925Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TAGGCAAGTCGAGGCAATGGATCGGAAGAGCACACGTCTGAACTCCAGTC11630.25443065944139015Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGAGAGACATTTAAGGTGAGGCCCAGTGGATCGGAAGAGCACACGTCT11310.24742998781445594Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGGTGAGGAGATAGAACAATGCCGATCGGAAGAGCACACGTCTGAACTC10780.23583512543234614Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCAAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACACGTCTGAAC10710.2343037285139543Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC10640.23277233159556243No Hit
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT10480.22927199578209537TruSeq Adapter, Index 1 (100% over 35bp)
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC10160.22227132415516115No Hit
TAGGCAAGTCGAGGCAATGGGGGATGGTGGTTGTTATGGTGATCGGAAGA9840.21527065252822694No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA9560.20914506485465947No Hit
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT9550.2089262938663178Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TAGGCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTC9540.20870752287797611Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTCTCAGTGTTTGCCCTATTGTGTGTTAAGGTGATGATCGGAAGAGCAC9530.2084887518896344No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT9020.19733143148420804No Hit
GCTGTGATGACTTGCCATTGATCGGAAGAGCACACGTCTGAACTCCAGTC9010.19711266049586632Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAGAGTAACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT7860.1719539968365715Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC7500.16407824125627052No Hit
GAGAGAACGCGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACA7220.15795265358270308No Hit
TGTCTCAGTGTTTGCCCTCACCAAAACTTTCTCGATCGGAAGAGCACACG7160.15664002765265292No Hit
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG6950.15204583689747736No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA6820.14920181404903532No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA6650.14548270724722653Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCAAGAAGCAGAAAGGGAAGAGCACACGTCTGAACTCCAGTCACATTC6470.14154482945707603TruSeq Adapter, Index 27 (97% over 34bp)
TGGACAGCAGGAATGTGTCAAGAAGAGATCGGAAGAGCACACGTCTGAAC6340.13870080660863401Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGAAGATCTTGACCAATTGCCAAAGTCATGATCGGAAGAGCACACGTC6310.13804449364360893No Hit
CAGAGTAGAAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAA6300.13782572265526724Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG6050.13235644794672488Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TGTCTCAGTGTTTGCCCATCGGAAGAGCACACGTCTGAACTCCAGTCACA6020.1317001349816998TruSeq Adapter, Index 1 (97% over 34bp)
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGCTGATCGGAAGAGC5870.1284185701565744No Hit
CTACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT5860.1281997991682327Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCATTCAAACCAGGAGGATACCGGATCGGAAGAGCACACGTCTGAACTC5850.12798102817989102Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTCTCAGTGTGTGGTCGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5850.12798102817989102Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTT5810.12710594422652424Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT5650.12360560841305714Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCAAGGGAGTGAAAATCTAGGATCGGAAGAGCACACGTCTGAACTCCAGT5630.12316806643637374Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG5480.11988650161124832No Hit
CACACCCCCAGGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTG5300.11594862382109784Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCA5160.11288582998431411Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TGTCTCAGTGTTTGCCCTATTGTGTATTTGCCGATCGGAAGAGCACACGT5140.11244828800763074No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT5130.11222951701928903No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT5110.11179197504260566No Hit
TGTCATAGCATTGTGTGTGGTCGATCGGAAGAGCACACGTCTGAACTCCA5040.11026057812421378Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTGTAGAAATGGCCGGATTGAAGAGCACACGTCTGAACTCCAGTCACATT5010.10960426515918872TruSeq Adapter, Index 8 (96% over 31bp)
CAGGTTATTGCGAGTGTTTTGATGGAATGATCGGAAGAGCACACGTCTGA4820.10544761638069654Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG4810.10522884539235483No Hit
TAGGCAAGTCGAGGCAATGGGGGATGGTGGTTGTTTAGAAGCCGATCGGA4730.10347867748562128No Hit
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG4670.10216605155557111Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTCTCAGTGTTTGCCCTATCGGAAGAGCACACGTCTGAACTCCAGTCAC4660.10194728056722943Illumina Multiplexing Index Sequencing Primer (96% over 33bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCT4650.10172850957888772TruSeq Adapter, Index 13 (97% over 37bp)
CCAAGGGAGTGAAAATCTGGGATCGGAAGAGCACACGTCTGAACTCCAGT4640.10150973859054603Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)