FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412476
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA231595.614629699667375TruSeq Adapter, Index 13 (97% over 38bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC217715.278125272743141No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG199894.846100136735228No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC182584.42643935647165No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT181944.4109233022042496Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC136703.314132216177426No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC124233.011811596311058No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA120022.909745051833319Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC113032.7402806466315615Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC95092.305346250448511Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC91482.217826006846459No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT77251.8728362377447416No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC76231.8481075262560729No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG75941.8410768141661575Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA72011.7457985434304057TruSeq Adapter, Index 13 (97% over 38bp)
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA67981.6480958892153725TruSeq Adapter, Index 13 (97% over 38bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG61401.4885714562786683Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA55421.3435933242176514No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA47341.147703139091729No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT45141.094366702547542No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC41411.0039371987703527No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT41180.9983611167680059Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGTGAAAGCATTGATGGAATGATCGGAAGAGCACACGTCTGAACTCCAGT35100.8509586012277078Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC34340.8325332867851705TruSeq Adapter, Index 13 (97% over 37bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC33210.8051377534692927Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC31500.763680795973584No Hit
GCTGTGATGACTTGCCATTGATCGGAAGAGCACACGTCTGAACTCCAGTC28650.6945858668140692Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT26060.6317943347006856No Hit
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT26000.6303397046131168TruSeq Adapter, Index 1 (100% over 35bp)
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC24060.5833066651150612No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC24050.5830642267671331No Hit
ACTCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGAACTCCAGT23700.5745788845896488Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT21140.5125146675200497No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG20490.49675617490472174No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC20320.49263472298994365No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA19780.479543052201825No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC19390.47008795663262837No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA18610.45117776549423483TruSeq Adapter, Index 13 (97% over 38bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC18340.44463193010017554Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT17770.43081294426827255No Hit
CAGAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAACTCCAG15750.381840397986792Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA15740.38159795963886384No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC14700.35638437145433915No Hit
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG14370.3483839059727112Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACA14070.34111075553486747Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG13950.33820149535973No Hit
TGTGAAGATCTTGACCAATTGCCAAAGTCATGATCGGAAGAGCACACGTC13700.332140536661527No Hit
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG12640.306442071781146No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC12630.30619963343321793No Hit
GTGATGACTTGCCATTGATCGGAAGAGCACACGTCTGAACTCCAGTCACA12620.30595719508528985Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GTGTAGAAATGGCCGGATTGAAGAGCACACGTCTGAACTCCAGTCACATT12310.29844160629951805TruSeq Adapter, Index 8 (96% over 31bp)
TAGGCAAGTCGAGGCAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTC11940.2894713874261775Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG11870.2877743189906807Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGAGAACGCGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACA11450.2775919083776996No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA11400.27637971663805894No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTTAAGGTGATGATCGGAAGAGCAC10790.26159097741444354No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA10020.2429232246239781TruSeq Adapter, Index 13 (97% over 38bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC9910.2402564027967688No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG9640.23371056740270948No Hit
TGGACAGCAGGAATGTGTCAAGAAGAGATCGGAAGAGCACACGTCTGAAC9490.23007399218378766Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT9300.22546766357315334No Hit
CATCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGCACACGTCTGAAC8770.2126184311329629Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG8610.20873941756611294Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGAGTAGAAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAA8510.20631503408683172Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CACACCCCCAGGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTG8500.2060725957389036Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC8330.20195114382412552No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT7870.19079897981943192Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGTCTCAGTGTGTGGTCGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7480.18134388425023518Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TTGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACACGTCTGAA7270.1762526789437446Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA7090.1718887886810384Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC7000.16970684354968532No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC6880.16679758337454786No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC6530.1583122411970636No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC6530.1583122411970636No Hit
CAGAGTAGAAGTGGTCAGACGACACAGGGATCGGAAGAGCACACGTCTGA6520.15806980284913547Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC6410.15540298102192612No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCT6410.15540298102192612TruSeq Adapter, Index 13 (97% over 37bp)
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA6280.15225128249886055No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG6040.1464327621485856No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAT5920.14352350197344815No Hit
GAGAGAACGCGGAATTGGTCAGGAGCCACTTGGATCGGAAGAGCACACGT5440.1318864612728983No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT4770.11564309196171413No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC4740.11491577691792977Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCTCTGATAGGAAAATGGATCCATTTTGTGGATGGGATCGGAAGAGCACA4740.11491577691792977No Hit
TAGGCAAGTCGAGGCAAGGTATTGATTGTATAGTGATCGGAAGAGCACAC4540.11006700995936733No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA4520.1095821332635111No Hit
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGCCGATCGGAAGA4450.10788506482801424No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGC4350.10546068134873303Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT4330.10497580465287676Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGGCAAGTCGAGGCAATGGATCGGAAGAGCACACGTCTGAACTCCAGTC4160.1008543527380987Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)