FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361270
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC196735.445511667174135No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC158054.374844299277549No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA155464.303152766628838TruSeq Adapter, Index 13 (97% over 38bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC138023.820411326708556Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC134983.7362637362637363No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT133733.7016635757189915Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC133223.6875467102167354No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG123223.4107454258587757No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA106742.954576909236859Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC101652.8136850554986577No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC89482.476817892435021No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG71331.9744235613253245Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC63011.7441248927395026Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC60901.6857198217399727No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC59221.6392172059678356No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT57001.5777673208403686No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA55611.5392919423146123No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC54961.521299858831345No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT53951.493342929111191No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT53391.4778420571871453No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC43121.193567138151521No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA42891.187200708611288TruSeq Adapter, Index 13 (97% over 38bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA42681.1813878816397707No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA42011.1628421955877875No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG41221.1409748941235087Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA40921.13267085559277TruSeq Adapter, Index 13 (97% over 38bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC38751.0726049768870929TruSeq Adapter, Index 13 (97% over 37bp)
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA37601.0407728291859275No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC33850.9369723475516926No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG31440.8702632380214244No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT29980.8298502505051623Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC21360.5912475433886013No Hit
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT21360.5912475433886013TruSeq Adapter, Index 1 (100% over 35bp)
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT20140.5574777866969303No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG20130.5572009854125723No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT20040.5547097738533507No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC17780.4921526835884519No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC15920.44066764469787134No Hit
TGTGAAAGCATTGATGGAATGATCGGAAGAGCACACGTCTGAACTCCAGT15210.42101475350845624Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC15010.4154787278212971No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC14690.4066210867218424No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA14430.39942425332853543TruSeq Adapter, Index 13 (97% over 38bp)
GTGTAGAAATGGCCGGATTGAAGAGCACACGTCTGAACTCCAGTCACATT14060.38918260580729097TruSeq Adapter, Index 8 (96% over 31bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT13740.3803249647078363No Hit
ACTCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGAACTCCAGT13570.3756193428737509Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC12890.3567968555374097No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC12410.3435103938882276Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GCTGTGATGACTTGCCATTGATCGGAAGAGCACACGTCTGAACTCCAGTC12330.34129598361336394Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACA10840.30005259224402797Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC10430.28870373958535167Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA9390.2599164060121239TruSeq Adapter, Index 13 (97% over 38bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT9270.25659479059982837No Hit
TGTGAAGATCTTGACCAATTGCCAAAGTCATGATCGGAAGAGCACACGTC8910.24662994436294183No Hit
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG8270.22891466216403245Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTCTCAGTGTTTGCCCTATTGTGTGTTAAGGTGATGATCGGAAGAGCAC7750.21452099537741853No Hit
CAGAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAACTCCAG7690.2128601876712708Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GAGAGAACGCGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACA7470.20677055941539568No Hit
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG7460.2064937581310377Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG7170.1984665208846569Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA7100.1965289118941512No Hit
GTGATGACTTGCCATTGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6890.19071608492263406Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC6450.17853682841088384No Hit
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG6240.17272400143936667No Hit
GAGAGAACGCGGAATTGGTCAGGAGCCACTTGGATCGGAAGAGCACACGT6210.1718935975862928No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG5960.16497356547734382No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC5880.16275915520248013No Hit
TAGGCAAGTCGAGGCAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTC5870.16248235391812219Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC5740.1588839372214687No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC5660.15666952694660502No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT5640.1561159243778891Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAGGTCAGGAGCCACTTGGATCGGAAGAGCACACGTCTGAACTCCAGTCA5560.15390151410302544Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGTCTCAGTGTGTGGTCGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5480.15168710382816175Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT5200.1439366678661389No Hit
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG4970.13757023832590584No Hit
CATCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGCACACGTCTGAAC4870.13480222548232626Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GCTCTGATAGGAAAATGGATCCATTTTGTGGATGGGATCGGAAGAGCACA4680.12954300107952502No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG4560.1262213856672295No Hit
CACACCCCCAGGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTG4550.12594458438287154Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCAGGATCGGAAGAGCA4530.12539098181415562No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC4450.12317657153929196Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGGACAGCAGGAATGTGTCAAGAAGAGATCGGAAGAGCACACGTCTGAAC4290.11874775098956458Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACT4250.11764054585213277No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT4220.11681014199905887Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA4220.11681014199905887Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG4210.11653334071470092No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA4100.11348852658676337No Hit
CAGAGTAGAAGTGGTCAGCCTCAAAAGGCTGCCACTTGCAAAGTTTCTTC4080.11293492401804744No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA3930.10878290475267804No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCT3910.10822930218396212TruSeq Adapter, Index 13 (97% over 37bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT3910.10822930218396212No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTT3850.10656849447781439Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTT3670.1015860713593711No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)