FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356548
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT4312912.096267543219987Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC213035.9747916129104635No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC178665.01082603183863No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT155684.366312530150218No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC149954.205604855447233No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC138373.8808239002883202No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA116653.2716492590058004Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG110903.110380650010658No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT109953.0837362711331995Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA106012.973232215578267TruSeq Adapter, Index 13 (97% over 38bp)
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT81992.29955013069769No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG77352.169413374917262No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG77342.1691329077711834Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC73032.0482515678113464No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC68731.9276506949975882No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT67281.8869829588162041No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA54971.5417279019935604Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC51511.4446862694503966TruSeq Adapter, Index 13 (97% over 37bp)
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT46621.307537835018006No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA40581.138135678786587Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC35280.9894880913649775Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA30930.8674848828208263No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC30350.8512177883482729No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA29150.817561730818852No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT28940.8116719207512032No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG26280.73706765989432Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC22630.6346971515756644Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA22250.6240394000246811TruSeq Adapter, Index 13 (97% over 38bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC21860.6131011813276193Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT21730.6094551084285986No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC21700.6086137069903632No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC21670.6077723055521276No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC21450.6016020283384004No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT17070.47875741835601376No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC15730.4411748207814936No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC14190.39798288028540335No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT13140.36853382994716Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC12760.3578760783961767No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC12540.3517058011824495Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT12390.34749879399127187No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA12280.34441365538440827No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC11260.31580600648440044No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC10350.29028349619125615No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC10310.28916162760694214No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT9970.27962574464027284Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA9480.26588285448242593TruSeq Adapter, Index 13 (97% over 38bp)
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC9180.2574688401000707No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC8980.25185949717850054Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA8460.2372752055824181TruSeq Adapter, Index 13 (97% over 38bp)
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA7850.22016670967162907No Hit
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG7740.2170815710647655Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC7380.20698475380593917No Hit
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG7320.20530195092946812Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT7280.20418008234515408Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA6800.1907176593333857TruSeq Adapter, Index 13 (97% over 38bp)
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6560.18398644782750148Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGTCTCAGTGTTTGCCCTATTGTGTGTTAAGGTGATGATCGGAAGAGCAC6450.1809013092206379No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG6370.17865757205200983No Hit
ATACCAGGGGCCATGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6050.16968262337749757Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG5910.16575608333239844No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT5640.1581834703882787No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT5620.1576225360961217No Hit
ATACCAGGGGCCATTGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTC5500.15425693034317958No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC5450.15285459461278705Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGTAGAAATGGCCGGATCCATTTTGTGGATGGGATCGGAAGAGCACACG5400.15145225888239452No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC4610.12929535434219236No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTT4380.12284460998238667No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC4260.11947900422944456No Hit
ACTCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGAACTCCAGT4220.11835713564513053Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGC4170.11695479991473798No Hit
GTGTAGAAATGGCCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT4090.11471106274610991TruSeq Adapter, Index 1 (100% over 35bp)
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA3960.11106498984708932Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGTCTGAACTCCA3940.1105040555549323Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACT3790.10629704836375468No Hit
CACACCCCCAGGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTG3690.10349237690296959Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA3690.10349237690296959No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGT3610.10124863973434153No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG3580.100407238296106No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)