FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497231
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT6165512.39966936896533Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT236614.758552865770637No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC206094.14475364568983No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA185273.726034780615046Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC173523.4897261031592963No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG165693.332254022778145No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC151763.052102543888052No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC128782.5899431049150197No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC128012.5744573447753663No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT94171.8938883537028062No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT93851.8874527131252878Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT83071.6706520711701403No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT75451.5174033799179858No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA72821.4645104589215074No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC72081.4496280400859962No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA70411.4160420408220726TruSeq Adapter, Index 13 (97% over 38bp)
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT69491.3975395741617076No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC63141.2698323314515787No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC62161.250123182182929No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC53881.083600982239643No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG53091.0677129945638948Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG49110.9876697148810111No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC47060.9464413924312844TruSeq Adapter, Index 13 (97% over 37bp)
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA46700.9392012967815764No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT46380.932765656204058No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC45380.9126542793993134No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC44260.8901295373779993Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG40700.8185330359531083No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC38850.7813269888643306No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA37490.7539755164098779No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA36490.7338641396051332Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC31710.6377317584784538No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC31170.6268716150038915No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT29370.5906711367553511No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG27800.559096275171902No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC27650.5560795686511903Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA27460.5522584070582888Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG25390.5106278570724673Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA24360.4899131389635803No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT23880.48025967809730286No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC22670.4559249121635618No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG22120.4448636549209522No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT22020.4428525172404778No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC21480.43199237376591565No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG21330.4289756672452039No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT19520.3925740752286161No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT19470.3915685063883788No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA18740.37688720132091524No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA17930.36059698610907204No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG16880.33948004046409014No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC16320.3282176694534331No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC16310.3280165556853857No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC14870.2990561730865533No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA13210.2656712875906772No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA13190.2652690600545823No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA12420.24978329991492887No Hit
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC12270.2467665933942172Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT12080.24294543180131567No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC11920.2397276115125565No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC11580.23288974339894333Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG11340.2280630129658046No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC10830.2178062107953848No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC10810.21740398325928995No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA10670.2145883905066257TruSeq Adapter, Index 13 (97% over 38bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC8970.18039904993855974Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT8640.173762295592994Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA8490.1707455890722823No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT7880.15847764922138804Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC7610.153047577484107No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC7460.1500308709633953No Hit
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7360.1480197332829208Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG7250.1458074818343989No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA6960.13997518256102293TruSeq Adapter, Index 13 (97% over 38bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT6570.1321317456071725No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGC6410.12891392531841336No Hit
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC6060.12187494343675273Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG6050.1216738296687053Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA5560.1118192550343804Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCATTGATCGGAA5500.1106125724260957No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT5380.10819920720952635No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA5380.10819920720952635TruSeq Adapter, Index 13 (97% over 38bp)
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA5320.10699252460124169TruSeq Adapter, Index 13 (97% over 38bp)
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG5150.10357359054443509No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCT5110.10276913547224528Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATACCAGGGGCCATTGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTC5100.10256802170419785No Hit
AAGCCCCAGTGATCTTCCTCCATCCTTTTATTTTGAGCCTATGTGTGTCT5030.10116022532786573No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)