FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557556
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT274534.923810343714353No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC245914.410498676366141No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT240194.307908084569084Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC218433.9176333857047543No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC207483.7212405570023463No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC181953.26334933172632No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG177053.1754657828092605No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC144692.595075651593741No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC130962.3488223604445113No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA128552.3055980027118355Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC125232.246052414465991No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT122802.202469348370388No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT121662.182022971683562No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA115852.0778181922533343No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC100761.8071727324250837No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG95721.716778224967537No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA95601.7146259747899764No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT86271.5472885234846365No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT84741.5198473337207383No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT82551.4805687679802566Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC81081.454203703305139No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG79831.4317844306222156No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA76671.3751085092797855No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA76051.3639885500290554TruSeq Adapter, Index 13 (97% over 38bp)
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT75611.3560969660446665No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT74251.3317047973656457No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC73341.3153835668524776No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT69581.2479463946222442No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA63391.136926156296408No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA62391.1189907381500692No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC61211.0978269447373896No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC59081.0596245040856882No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG53590.961159058462289No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA48930.8775800099003509No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA47320.8487039866847454No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG46700.8375840274340155No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG44830.8040447955003622No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC43070.7724784595628063No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG41690.7477275825208589No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT41060.7364282690886655No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC40940.7342760189111049No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG40940.7342760189111049Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC39600.7102425585950112TruSeq Adapter, Index 13 (97% over 37bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT34940.626663510033073No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA34800.6241525514925855No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC31790.5701669428721061No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA29510.529274189498454Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT29480.5287361269540638No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC21540.38632890687213484No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA21320.3823831148799403Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG18750.3362890902438499Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC18380.3296529855297047Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC17360.3113588590204392Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA16090.2885808779745891No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC15440.276922856179469Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA14990.2688519180136166TruSeq Adapter, Index 13 (97% over 38bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC14920.26759643874337286No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT14880.2668790220175193No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC14310.25665583367410627No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG13190.23656816535020697No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA13110.2351333318984999No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA13110.2351333318984999No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC12450.22329595592191637No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC11090.1989037872428958No Hit
TGTCTCAGTGTTTGCCCTATTGTGTGTGGTCGATCGGAAGAGCACACGTC11000.1972895996097253No Hit
GTGTATTTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA10900.19549605779509144TruSeq Adapter, Index 13 (97% over 38bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT10610.19029478653265322No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG10220.18329997345558113No Hit
TGTCTCAGTGTTTGCCCTATTATTGGATCGGAAGAGCACACGTCTGAACT10210.18312061927411777Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC9700.17397355601948505No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC9030.16195682586143811Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC8080.1449181786224164Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC7670.13756465718241756No Hit
CAGAGTAGAAGTGGTCAGACACCCAAAAGTCCACCTGAACACTATGTTCA7410.13290144846436952No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGC6980.12518921866144386No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT6920.12411309357266355No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA6630.11891182231022533TruSeq Adapter, Index 13 (97% over 38bp)
CAGAGTAGAAGTGGTCAGCCTCAAAAGGCTGCCACTTGCAAAGTTTCTTC6520.1169389263141281No Hit
TGTCTCAGTGTTTGCCGATCGGAAGAGCACACGTCTGAACTCCAGTCACA6330.11353119686632375Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCC6260.11227571759608004No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTT6210.1113789466887631No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT6020.10797121724095875Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC5610.1006176958009599No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)