FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457997
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC301006.57209545040688No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC283726.194800402622724No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC239335.225580080218866No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC226254.939988689882248No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT218714.775358790559763No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG192814.209852902966613No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT173093.7792823970462686No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC154143.3655242283246394No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC131032.86093577032164No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT128992.816393993847121Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT120422.6292748642458355No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG114682.503946532400867No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC97952.138660296901508No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC95172.0779612093528996No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT95092.0762144730205656No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA94422.061585556237268No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT83691.8273045456629629No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA80851.765295405865104No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT80531.7583084605357675No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA79471.7351642041323414No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC72591.5849448795516128No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT68871.5037216400980793No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC67721.4786123053207771No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA67721.4786123053207771Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT65991.4408391321340535No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC61271.337781688526344No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG57401.253283318449684No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC52811.153064321382018No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA51611.126863276397007No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA46841.022714122581589No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC44450.9705303746531091No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA40690.8884337670334086No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG34850.7609220147730226No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA31940.6973844806843713No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT27440.5991305619905807No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG25280.5519686810175612No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG22750.4967281445074968No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA22150.4836276220149914Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC20830.45480647253147943No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG18520.40436946093533366No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG18070.3945440690659546Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA16550.3613560787516075No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT15960.3484738983006439No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT15900.34716384605139333Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA15850.34607213584368457Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATT11980.2615737657670247TruSeq Adapter, Index 7 (97% over 35bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAT10350.2259840129957183TruSeq Adapter, Index 7 (97% over 35bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC9600.20960835988008655No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC9300.20305809863383384Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT9080.1982545737199152No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC7900.1724902128179879No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT7830.1709618185271956No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC7280.15895300624239897No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT7270.15873466420085722No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTT7140.15589621766081438No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACT6760.14759922008222762No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC6580.143669063334476No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGT6180.13493538167280572No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA6170.13471703963126397No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG6160.13449869758972222No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC6050.13209693513276288No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGC5650.1233632534710926No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA5130.11200946731092125No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC5040.11004438893704545No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)