FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772180
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC430075.569556321065037No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC354144.586236369758347No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT339214.392887668678287No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT291583.7760625760832967No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA242823.1446035898365663No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC232013.004610324017716No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC220802.8594369188531172No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT212612.7533735657489187No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG208952.7059752907353207No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT170732.2110129762490613No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA170392.2066098578051747No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT169342.1930119920225857No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC165992.1496283250019426No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC156862.031391644435235No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG148551.9237742495273122TruSeq Adapter, Index 8 (97% over 36bp)
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG140981.8257401124090238No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC140091.8142143023647337No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT134511.7419513584915434No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA126211.6344634670672642No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC123091.5940583801704267No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT120401.5592219430702685No Hit
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT117941.527364086093916Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT108761.4084798881089902No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC108261.4020047139268046No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC105611.3676862907612215No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC103961.3463182159600093No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC90341.1699344712372763No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA89421.158020150742055No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA84271.0913258566655444No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTC77891.0087026341008574TruSeq Adapter, Index 8 (97% over 36bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC77000.997176824056567No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC75690.9802118676992411No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC72310.936439690227667No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA58090.7522857364863115No Hit
TAGGCAAGTCGAGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGT55550.7193918516408091No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA55280.715895257582429Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT54310.7033334196689891Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG53120.6879225051153877No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG52230.6763966950710975No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC45350.587298298324225Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG43770.5668367479085187No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC43710.5660597270066564No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA43190.5593255458571836No Hit
CAGAGTAACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT42470.5500012950348364Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38070.49301976223160404Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC37370.48395451837654435No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA37220.4820119661218886No Hit
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC36790.4764433163252092Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT36780.47631381284156543No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA31490.40780646999404285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG31080.40249682716465074No Hit
TTAACAGATGATTTTATTCCTAGATCGGAAGAGCACACGTCTGAACTCCA31030.4018493097464322Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTACTGAAGTGGGATGCCACTCTTAGGGTTTGGGATATTGAGACAGGCCA30830.39925924007355795No Hit
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG29820.38617938822554326TruSeq Adapter, Index 8 (97% over 36bp)
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA28820.3732290398611723No Hit
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA27460.3556165660856277Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA26170.33891061669558914No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC26060.3374860783755083Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG23800.30821829107202986No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC22270.28840425807454223No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT21980.2846486570488746No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG20870.2702737703644228Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGTCTGAACTCCA16160.20927762956823537Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
ACAAACTGGTGGTGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTC14150.18324742935584967Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATACCAGGGGCCATGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACT13760.17819679349374498Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT13000.16835452873682302Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGCCGATCGGAAGA12310.15941878836540702No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT11850.15346162811779637No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC11650.15087155844492217Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTCTGAACTCCA10710.13869823098241343Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG10530.13636716827682666TruSeq Adapter, Index 8 (97% over 36bp)
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT10420.13494262995674583No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAT9880.1279494418399855No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC9790.1267839104871921No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT8810.11409256909010851Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA8800.11396306560646481No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCC8360.10826491232614158No Hit
GCTCTGATAGGAAAATGGATCCATTTTGTGGATGGGATCGGAAGAGCACA8180.1059338496205548No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)