FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637747
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC278554.36771948750837No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA224913.5266336023532845No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA219033.4344340310499306No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC204443.205659924703683No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT202773.179473992037595No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC194033.0424290510186642No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC184932.899739238287283No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG172982.712360857832338No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC171802.693858222774862No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT170292.6701811219809737No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC145102.2751969041014695No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT128462.014278389392659No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC119491.8736270025574406No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT116711.8300360487779637No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG115771.815296661528788TruSeq Adapter, Index 8 (97% over 36bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC115351.8087109778642627No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT114721.798832452367475No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG112251.7601023603403858No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA103601.6244686372495678No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC99671.5628454543886525No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA97671.5314850559861513Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA97151.5233313524015009No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC95551.4982430336794998No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT94081.4751931408536614No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG86471.355866824932144No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT85321.3378345958507056No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC82131.2878147603987162No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC81421.2766818189658282No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT77151.2097273683764878No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA76291.1962423970634124No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC76151.1940471691752372No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTC75201.179150979934049TruSeq Adapter, Index 8 (97% over 36bp)
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT71241.1170573910970965Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC68961.081306536918245No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG66701.0458692867234185No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA62830.9851869158145785No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA61060.9574329632283649No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC53210.8343433994985472No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC50840.7971813273915832Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA50720.7952997034874331No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC46710.732422104690418No Hit
TAGGCAAGTCGAGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGT45230.7092154098725669No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG44880.7037273401521293No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA43830.6872631309908162Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC43690.685067903102641Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC42790.6709557238215154No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT37600.5895754899670245No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG37570.589105083990987No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG31820.4989439385837958No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT29200.45786181667651904Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CTACTGAAGTGGGATGCCACTCTTAGGGTTTGGGATATTGAGACAGGCCA28260.4431224294273435No Hit
CAGAGTAACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT27210.42665822026603023Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT24090.3777359987581282No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC23030.36111498760480254No Hit
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA22170.34763001629172696Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA21870.34292595653135177No Hit
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC21250.33320423302657637Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC19810.31062474617677543Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTAACAGATGATTTTATTCCTAGATCGGAAGAGCACACGTCTGAACTCCA18760.29416053701546224Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCATCATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG17940.2813027736704367TruSeq Adapter, Index 8 (97% over 36bp)
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT16020.2511967912040355Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGCCGATCGGAAGA15640.2452383155075602No Hit
ACAAACTGGTGGTGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTC14580.22861730435423452Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG13910.21811157088939656Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCATCTTTGTCATCAGCTGAAGATTAAATAGGATGTAATCAGACGACAC12970.20337218364022097No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA12800.20070654977600838No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT12560.1969433019677082No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA11910.18675117248689527No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG10660.16715092348533195Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGTCTGAACTCCA10180.15962442786873166Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC9910.15539077408439397Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAGGAGAGACATTTAAGGTATTGATTGTATAGTGATCGGAAGAGCACACG9470.14849148643584367No Hit
CTACTGAAGTGGGATGCCAGCCCAGGGCTGCTAGCACACCGGGGCCTGTG9420.14770747647578114No Hit
CCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG8660.13579052508283065TruSeq Adapter, Index 8 (97% over 36bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG8360.13108646532245546No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTT7470.11713108803334238No Hit
CAGAGTAGAAGTGGTCAGCCTCAAAAGGCTGCCACTTGCAAAGTTTCTTC7100.11132941432887963No Hit
ATACCAGGGGCCATGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACT7100.11132941432887963Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CAGAGTAGAAGTGGTCAGACGACACAGGGATCGGAAGAGCACACGTCTGA6750.1058413446084419Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAGAGTAGAAGTGGTCAGACACCCAAAAGTCCACCTGAACACTATGTTCA6550.10270530476819177No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT6460.10129408684007922Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTCTGAACTCCA6430.1008236808640417Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)