FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002318895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816015
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC10674813.081622274100354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8622710.566840070341845No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG792299.70925779550621No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT629897.719098300889077No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA562746.896196761088951No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA548236.718381402302653No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT470345.763864634841271No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA436805.3528427786253925No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA407814.997579701353529No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC359794.409110126652084No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA355454.355924829813177No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT307753.7713767516528494No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT250843.0739631011684834No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT149401.8308486976342346No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG83771.0265742664044166No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC58720.719594615295062No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG49570.6074643235724833No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC46430.5689846387627678No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA25730.3153128312592293No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA20610.25256888660134924No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA16370.2006090574315423No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG16050.1966875608904248No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA15060.18455543096634253No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC9520.11666452209824574No Hit
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGC8900.10906662254983057No Hit
TGTCTCAGTGTTTGCGCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC8190.10036580209922613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)