FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591171
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10858818.368289378200217No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA296815.020713126997096No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG240994.076485483895523No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA237314.014236151638021No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC228533.86571736434974No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA213733.615366788966306No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT213423.610122959346788No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG182493.0869240879542468No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA181003.0617198746217253No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC149442.5278641881959705No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC143322.4243408421590367No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA131932.2316723925902995No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT120182.0329143344311547No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG119152.015491287630821No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT118982.0126156391297947No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT96111.6257563378447182No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT94171.5929401137741872No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA92251.560462201292012No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC91931.555049215878316No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC89621.515974227423199No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG88671.4999044269762893No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT85171.4406998990139908No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG78211.3229674662661057No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC62751.061452608466924No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT60981.0315120328974188No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC59761.0108750260077035No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA59020.9983574972385316No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG57030.9646954941971104No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC55350.9362773207752072No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC54450.9210532992991874No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC52660.8907744121413264No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC51100.864386108249559No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA49110.8307241052081378No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA47600.8051815802872604No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA43770.740394911117088No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC40310.6818670063315013No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC38270.6473592243191901No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT37240.6299361775188567No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT34160.5778361929120339No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA33630.5688709358206001No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA29310.4957956327357059No Hit
AACATCACCACGTCTCTGGGCCAGACAGCAGAACTGCACTGCAAAGTCTC29070.491735893675434No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA25440.4303323403888215No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC21240.3592869068340632No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC19870.3361125630316778No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG17180.2906096543977969No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC17020.287903161690949No Hit
GAATCCACCTCCCACCATCCGCTGGTTCAAAAATGATGCTCCTGTGGTCC16760.28350511104232107No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG14270.24138531829200013No Hit
ATCACCATTGTCCAAGGCCAGACGGCAATTCTGCACTGCAAGGTGGCAGG9520.16103631605745208No Hit
ACAAACTGGTGGTGGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACG8930.15105612420095033No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA8790.14868794308245836No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCAT7940.13430970057732874No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT6540.11062788939240929No Hit
CCCCAGAGGCCATCATGTACGGCAAGTTCTCCATCGACTCAGACATCTGG6240.10555321556706942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)