FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376102
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT13318335.41140435307443No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4685212.457258935076123No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG204035.424858150182663No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC191645.095426240753838No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC137683.660709062966961No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC119803.18530611376701No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC101632.7021924903350687No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA74871.990683378445209No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC59481.5814858735130364No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG42301.1246948965971997No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA38541.024722016899671No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA37691.002121764840389No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT34680.922090284018697No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA33130.8808780596753009No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA29930.7957947578050635No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA28730.7638885196037245No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG25920.6891747451489224No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA24830.6601932454493727No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC24800.6593955894943393No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT24150.6421130438019473No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA23930.6362635667983685No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG23850.6341364842516126No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG21280.5658039574370782No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT21020.5588909391601214No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA20560.5466602145162748No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT19810.526718815640438No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA16980.45147327054894687No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC15140.4025503719735603No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC13750.36559231272367604No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC12510.3326225332489591No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG12400.32969779474716965No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA11700.3110858224630552No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA11650.30975639587133275No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG11240.29885509781920866No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT10770.2863584878570175No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC9160.24355095160355436No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT9100.2419556396934874No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT8700.23132022695970772No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC8190.2177600757241387No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT8040.21377179594897128No Hit
GGATTCGAGAAGTGACAGGCTATGTCATCGTGGCCATGAATGAATTCTCT7860.20898586021877041No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG7500.19941398875836874No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG6910.18372675497604374No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC6810.18106790179259882No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTTGCCATGAATGAATTCTCT6680.1776113926541204No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC6270.16671009460199626No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTATCT6050.16086061759841747No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGT5750.15288405804808272TruSeq Adapter, Index 2 (96% over 32bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC5450.14490749849774795No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA5220.13879213617582464No Hit
GGATTCGAGAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCTA5050.1342720857639683No Hit
GCGTGGCTTTCAACAAAGAGCACACGTCTGAACTCCAGTCACCGCTCATT4960.13187911789886786TruSeq Adapter, Index 2 (96% over 31bp)
GGATTCGAGAAGTGACAGGCTATGTTCTCGTGGCCATGAATGAATTCTCT4490.11938250793667673No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCAT4460.11858485198164327No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATTAATTCTCT4080.1084812098845526No Hit
CTACTGAAGTGGGATGCCATTGTCACAGAGGATAA4020.10688589797448564No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCAATGAATGAATTCTCT3960.1052905860644187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)