FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738954
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14198619.21445719219329No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT7544010.209025189659979No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC399195.402095394300592No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA246883.3409386781856516No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG230573.1202212857633898No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA193942.624520606154104No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC175172.3705129142003423No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC170322.304879600083361No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG161292.1826798420470013No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC156672.1201590356097944No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA155852.109062269099294No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC131481.7792717814640695No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG119951.623240418212771No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG109211.4778998422093932No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT102871.392102891384308No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC101341.3713979489927655No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT100731.3631430373203202No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA96591.3071178990843815No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGT95931.2981863553076376TruSeq Adapter, Index 7 (96% over 33bp)
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA87621.1857300995731805No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT85791.160965364555845No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC83501.129975614178961No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC82391.1149543814635283No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA79831.0803108177234308No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG76721.0382243008360466No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG73610.9961377839486627No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA73330.9923486441645893No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT72010.9744855566111016No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT71180.9632534636797419No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT70690.9566224690576138No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC64660.8750206372791811No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC63800.8633825650852421No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT62390.8443015397440166No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA62190.8415950113268215No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG61570.8332047732335165No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC59800.8092519967413397No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG59630.806951447586724No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA58220.7878704222454983No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA57630.7798861634147728No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT52730.7135762171934924No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA49490.6697304568349316No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC44850.6069389975560048No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA41180.5572742011004744No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA41100.5561915897335964No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC34060.4609217894483283No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC30790.4166700498271882No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCAT25210.3411579069874444No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT14270.19311080256687155No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAACTCGTATGCCGT13510.18282599458153012TruSeq Adapter, Index 7 (96% over 33bp)
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC11890.16090311440224966No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGC8730.11813996541056683TruSeq Adapter, Index 7 (97% over 36bp)
GGCCTCTGATTCCTCACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG7840.10609591395404858No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCATGGCCATGAATGAATTCTCT7750.10487797616631075No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC7690.10406601764115223No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCA7520.10176546848653636No Hit
TCGGGAAACCTGCAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT7430.10054753069879856TruSeq Adapter, Index 9 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)