FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665672
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG9715814.59547645086469No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9411514.13834440985951No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA517187.769291783340744No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA483567.264238243459241No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA478387.186422141835619No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT458866.893184631470153No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA418126.281171507889772No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC411366.1796199930296005No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA380195.711371366078188No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT315024.73236068213775No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG190742.865375139708445No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC136032.043498900359336No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA117611.76678604477881No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA110691.6628309437681021No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG50240.7547260512684926No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG47240.7096588109459313No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG32300.4852239541395763No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG30820.46299078224711265No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC30540.4587845064836737No Hit
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGC17380.26108954560203823No Hit
ACAAACTGGTGGTGGTTGGAGGTGAGGAGATAGAACAATGC10120.15202682402144No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC9890.14857166893004362No Hit
AACATCACCACGTCTCTGGGCCAGACAGCAGAACTGCACTGCAAAGTCTC9890.14857166893004362No Hit
ACAAACTGGTGGTGGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACG7480.11236765253758607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)