FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002318919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578665
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8989715.53524059689112No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC7314212.639782948683608No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC6604311.412993701018724No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC528849.138966414073774No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC429927.429514485928819No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT259144.478238704604564No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT212923.6795036852064666No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG193443.3428667709296396No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT159362.75392498250283No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC89971.554785584059862No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT88151.5233338805699326No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA79971.3819740264228872No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT72031.2447616496591292No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT64221.109795823144652No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG61441.061754210121573No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA59011.019761001615788No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG55910.9661894187483259No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC53580.9259243258189107No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC52900.9141731398995965No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG44850.7750598360018318No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG41530.7176863988663562No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA33730.5828933839095158No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT32120.5550707231299629No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC31170.5386536251544503No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC31050.5365798864628066No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC30300.5236190196400335No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT30190.5217180925060269No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC26430.45674094683452426No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA26430.45674094683452426No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA22570.390035685586652No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA22200.383641657954084No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA21150.36549644440220164No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC20120.34769685396559324No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG19690.3402659569872033No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA19090.32989726352898485No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT16310.28185565050590583No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC15080.260599828916558No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC14910.25766203243672936No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA14560.25161362791943526No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG13510.2334684143675529No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC12420.21463195458512266No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC10000.17281155763697476No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT9230.15950506769892772No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG8010.1384220576672168No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG7980.13790362299430586No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA6740.11647498984732099No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA6390.11042658533002686No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA5990.10351412302454788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)