FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318566
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7981825.05540453155704No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA226707.116264761462303No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG187965.900190227456791No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG187835.896109440429926No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC171285.376593861240685No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC170205.342691938248275No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG164655.168473722870614No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA114273.5870117966135746No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT100943.1685741730128134No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA95693.0037731584663776No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC86222.7065035188940443No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA65042.041649140209564No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG52321.6423598249656273No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC48141.5111468267172266No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA45401.4251363924587055No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC45311.422311232209338No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT38341.2035182662305457No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT32011.0048153286916996No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA30530.9583571379243233No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG20360.6391140297457983No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA16910.5308162201867117No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC15950.5006811775267919No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC15110.47431301519936214No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC13310.4178098102120126No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA11930.3744906863883779No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC11490.36067879183591467No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT11410.3581675382809214No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT11010.3456112705059548No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA10590.3324271893422399No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC10460.32834640231537576No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG9980.3132788809854159No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT9940.31202325420791927No Hit
ACAAACTGGTGGTGGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACG9140.2869107186579861No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT9050.28408555840861865No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT8740.2743544508830195No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG8520.2674485036067879No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA8160.25614786260931804No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT8140.2555200492205697No Hit
ACAAACTGGTGGTGGTTGCCCCAGCTTCCAGACAGGAAGACGGCTACAGA7680.24108034127935815No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC7550.236999554252494No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG7300.2291518868931399No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA7000.21973468606191496No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA6760.21220092539693503No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA5430.17045133504517115No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT4900.15381428024334046No Hit
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGC4260.13372425180339395No Hit
GGCCTCTGATTCCTCACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG3740.11740110369593741No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC3590.11269250328032494No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA3550.1114368765028283No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC3500.10986734303095748No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC3210.1007640488941067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)