FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449273
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24927555.484082061463745No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC84611.8832647410371866No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA84341.877255032018394No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG73171.6286311440927899No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC70941.5789953992338734No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT69171.5395984178884552No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC68771.530695145268022No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG67601.5046530728532541No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA57261.2745034756150493No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT57041.269606675673811No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC52361.1654383860147393No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA44040.9802503155097234No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA44030.9800277336942127No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA43130.9599953702982373No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG42310.9417436614263488No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC41810.9306145706508069No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA38580.8587206442408069No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA37460.8337914809035932No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC37340.8311204991174631No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT34760.7736943907156674No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC34610.7703556634830048No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC32970.7338522457392276No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA31480.700687555228113No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC30320.674868064628856No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT30090.6697486828721068No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC29590.6586195920965648No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC27850.6198903561976794No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG26830.597187011015574No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT25600.5698094477077411No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT24650.5486641752342117No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT23730.5281866482072147No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT23720.5279640663917039No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG22600.5030349030544903No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC21660.4821122123964717No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA20650.4596314490298772No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG19740.439376503818391No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC18490.4115537768795365No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG18360.4086602132778956No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC18050.4017601769970597No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA18030.40131501336603803No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC16010.3563534866328491No Hit
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGC14190.31584359620987684No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT13630.30337901454127No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT13170.2931402510277715No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA13130.29224992376572817No Hit
ACTCAAGAAGCAGAAAGGGAAGAGCACACGTCTGAACTCCAGTCACATTA12250.2726627240007746TruSeq Adapter, Index 27 (96% over 33bp)
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA11680.25997556051665693No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC10920.24305934253783332No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG10750.23927545167414913No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC10300.2292592699761615No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG9890.2201334155402172No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG8460.1883042159221676No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA8370.18630097958257005No Hit
CTACTGAAGTGGGATGCCATTGTCACAGAGGATAA6830.15202337999390125No Hit
CTACTGAAGTGGGATGCCAGCCCAGGGCTGCTAGCACACCGGGGCCTGTGGGTGGAGTCACAGGTAAGTGC5020.11173607138643987No Hit
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGC4600.10238763513498474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)