FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002318941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045511
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC574915.498842192956363No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT447384.279055887503814No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT409053.9124409021043296No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN383673.6696887933268996No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC368923.528609455089425No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG332963.1846628108169113No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT319763.0584087589704936No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA317773.0393750041845564No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA315263.0153676049319422No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC306562.9321547071240763No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA293532.807526654430226No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA283382.710444940321049No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC278182.660708495654278No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC274722.627614630549081No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA264542.53024597541298No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA257602.463866951184636No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG246992.3623854746626294No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT224972.1517707609006504No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA217932.084435266582561No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT211072.018821418426014No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC202641.9381909898604606No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT191071.827527400476896No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC187781.796059534524266No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA184721.766791549778051No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT184281.7625830813831707No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC180261.7241329837753976No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC179441.716289929039484No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG176811.6911347656791749No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC173801.6623450159778328No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG158681.5177267384083No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG153801.4710509980287152No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC151091.4451306585966097No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA146591.4020895045580584No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC142911.3668914052554204No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT141131.349866237657949No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC132601.2682793390026503No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG129991.2433154696602906No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC122381.1705280958306512No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT102960.984781604402058No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC70630.6755548243873092No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG51630.49382550733564734No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG30070.28761055598649843No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT27960.2674290370928666No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT24820.2373958762748551No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA24560.23490905404151657No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC18330.17532096745036638No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT18060.1727384982080533No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA16390.15676544770930195No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG12870.12309770055025725No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG12560.12013264327204592No Hit
GGCCTCTGATTCCTCACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG11320.10827241415920061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)