FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002318950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165892
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30320226.00601084834616No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC961858.249906509350781No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT748866.4230649151036285No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC713886.123037125222576No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG661525.673938924016976No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT618165.302034836845952No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT604355.1835847574217855No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC524654.499987992026706No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT505034.3317048234313305No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC427033.662689168465003No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC357353.065035183361752No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT285032.4447375914750253No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG207581.7804393545885897No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC191331.6410610931372718No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG144801.2419675235785133No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA135411.1614283312691054No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT97690.8378992222264156No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA95730.8210880596144412No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT86880.745180514147108No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA79430.6812809419740422No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC71570.6138647490505124No Hit
GCTCTGATAGGAAAATGAGATCTACTGCTGTGATGACTTGCCAT40520.34754505563122484No Hit
TGTCTCAGTGTTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGTG37510.32172791304855No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA34750.29805505141127997No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA28850.2474500210997245No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCA24200.2075663955151935No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA19940.17102784820549416No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA15550.13337427480418426No Hit
GTGTAGAAATGGCCGGATTGAAGAGCACACGTCTGAACTCCAGTCACGAG15150.12994342529153644TruSeq Adapter, Index 7 (96% over 31bp)
TCATCATTAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAG12830.11004449811817904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)