FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002318952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039635
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18739518.025076108441908No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT1013379.747363257297032No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG832008.00280867804566No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT710516.834225473363248No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT674556.4883348482881No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC632746.086174474695446No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT580635.584940868670254No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC499624.80572508620814No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC379553.6508005213368153No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC295742.8446522096697398No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC281352.706238247077099No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT247482.38045083130137No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG181731.7480173330062954No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA174251.6760690049873275No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT155721.4978333742130652No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG153091.4725360342812621No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT151411.4563765167582854No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC129301.2437057236433942No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA122231.1757010874008667No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC106751.0268026759391518No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA88960.8556849278833437No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA80750.7767149047502249No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA60010.5772218134249039No Hit
GTGTAGAAATGGCCGGATTGAAGAGCACACGTCTGAACTCCAGTCACATT45540.4380383499978358TruSeq Adapter, Index 8 (96% over 31bp)
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA28820.27721267560249513No Hit
GCTCTGATAGGAAAATGAGATCTACTGCTGTGATGACTTGCCAT23040.2216162403151106No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG14930.1436080932250261No Hit
ATCCAGACTGTGTTTCTCGCTTCTCAGGATTCCTACAGGAAGCAAGTAGT12910.12417819715573254No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA10810.10397880025201152No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCA10550.10147792254012226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)