FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273972
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13403748.92361263194779No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC111194.058443928576643No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC99133.618252960156512No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC88373.2255120961266113No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT84813.0955718102579826No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC65742.3995152789336136No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT64362.34914516811937No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG63392.3137400902281984No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT52011.8983691764121882No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT43251.5786284729826405No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG41671.5209583461083613No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA39271.4333581533879374No Hit
GTGTAGAAATGGCCGGATTGAAGAGCACACGTCTGAACTCCAGTCACCGC38711.4129181084198386TruSeq Adapter, Index 11 (96% over 31bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC36451.3304279269414392No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG34251.2501277502810506No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC34211.2486677470690437No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT33581.2256726964799323No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA29941.092812404187289No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA28761.0497423094330807No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC28701.04755230461507No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC27060.9876921729227803No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT25680.9373220621085367No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA20630.7529966565926446No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC17470.6376564028440863No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA16570.6048063305739273No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT14900.5438511964726322No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC14440.5270611595345509No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA14420.5263311579285475No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA7800.28470062634137794No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG6670.24345553560217834No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCA6010.21936548260406172No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA4770.17410538303184267No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA4610.1682653701838144No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT4550.1660753653658038No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCA3630.1324952914896413No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG3180.11607025535456178No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT3080.11242024732454413No Hit
GCTCTGATAGGAAAATGAGATCTACTGTGATGACTTGCCAT2960.10804023768852293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)