FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317714
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11508536.22282933707674No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT147954.656703827971068No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG114843.6145715958377664No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC104543.290380656817137No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC73232.3049031518913234No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG73022.2982934337171166No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT71902.2630416034546794No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC70982.234084742881963No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT69672.192852691414291No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT68882.1679875611398933No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA68862.167358064170921No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT67192.1147950672617513No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC64392.026665491605658No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT61041.921224749302832No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA60881.9161887735510554No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA59541.874012476629925No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG56901.7909188767256086No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA54561.7172677313558735No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA53561.6857928829072686No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC52921.665648979900162No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT47601.4982027861535847No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC38101.1991917258918399No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA36701.1551269380637932No Hit
TTAACAGATGTTTTACTGTGCTCCCTAAACACTACCAAA25050.7884449536375482No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG24300.7648388173010947No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA21430.6745060022535991No Hit
GTGTAGAAATGGCCGGATTGAAGAGCACACGTCTGAACTCCAGTCACTAA20340.64019841744462TruSeq Adapter, Index 3 (96% over 31bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA18250.5744159841870361No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC17170.5404231478625431No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA12000.37769818138325667No Hit
CATCTCCTACTACGAGCTGAACCAACATCATCTTCTCAGA10090.31758122084642165No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG9070.2854768754288448No Hit
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGC8600.27068369665800057No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA8120.25557576940267035No Hit
CTGTTTCGGTTGGCTTTGGAGTCACAGGTAAGTGC7610.23952359669388193No Hit
GACCTCGTCTTCTACTTCTGGCTCCCTAAACACTACCAAA7170.22567466337649586No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC6610.2080487482452772No Hit
GACCTCGTCTTCTACTTCTGGATCCCTAAACACTACCAAA6050.19042283311405855No Hit
CTACTGAAGTGGGATGCCAGCTCCCTAAACACTACCAAA5360.1687051876845213No Hit
CTGTTTCGGTTGGCTTTGGTTGGAGTCACAGGTAAGTGC4870.153282511944705No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC3880.1221224119805863No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG3380.10638498775628397No Hit
TTAACAGATGTTTTACTGTGCTCCCTACACGACGCTCTTCCACACAATTAAACAGCATG3310.10418174836488163Illumina Single End PCR Primer 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)