FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402638
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN353918.789781391721597No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC181074.497091680367974No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT163724.06618351968766No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC143563.5654856223207942No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG141853.523015711383427No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC129033.204615560379299No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT125153.108251084100358No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA116862.902358942772416No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG115102.8586472215737215No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT114442.842255326124211No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA110772.751106452942842No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA110172.736204729806923No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC104812.603082669792717No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA101442.5193846581793076No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA97962.4329546639909796No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT97752.427739060893408No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC96082.3862625981651013No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC95942.3827855294333866No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA82862.057927965070361No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT79971.9861513319656865No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT77531.9255509912129505No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG76131.890780303895807No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC76001.8875515972163581No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT75701.880100735648399No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC70681.7554229854112129No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA64651.6056606678952308No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC63141.5681579980031692No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC62501.5522628266581893No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC60951.5137667085570663No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG57081.4176505943303912No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA56961.4146702497032073No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA55401.3759257695498188No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG55391.3756774074975535No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG53891.338423099657757No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT51201.2716137075983887No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC50141.2452873300582656No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC48681.2090264704275304No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT35440.8801951132282597No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG24270.6027747008479081No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC11670.2898385149936171No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG9880.24538170763812656No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG9390.23321196707712633No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA8820.21905533009800365No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT8050.19993145207357477No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT5760.14305654210481872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)