FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002318967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144103
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25572922.351921111997783No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC518864.535081194612723No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG440533.8504400390524283No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT400493.5004715484532425No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT357873.1279526406276354No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT332382.905158014619313No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA319682.794154022845845No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA281502.4604428097819864No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA273112.387110251437152No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA242682.1211376947704883No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC226441.9791924328491404No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC225161.9680046289538617No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA223241.9512229231109437No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC217441.9005281867104622No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA215061.8797258638426784No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA210451.8394322888760888No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC207831.8165322527779404No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA199931.7474825256117674No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC195351.7074511647989736No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT179191.5662051406210804No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG178541.5605238339555092No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG171681.5005641974542503No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC168301.47102140279328No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG158051.3814315669131187No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA143641.2554813683733022No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT143311.2525970126815504No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG137231.1994549441789768No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC136191.190364853514063No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT123921.0831192646116652No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC123501.079448266458527No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG112320.9817297918107024No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC101990.8914407181870863No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT100130.8751834406517596No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC99970.8737849651648496No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC95150.8316558911216909No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC85840.7502820987271251No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT84940.7424156741132573No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT77420.6766873262284951No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC75350.6585945496165992No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA56360.4926129902639884No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG46750.40861705633146667No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC35860.3134333185036662No Hit
TTAACAGATGTTTTACTGTGCTCCCTAAACACTACCAAA34030.29743825512213495No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG29430.25723208487347726No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC28180.2463064951319942No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT27080.23669197615948911No Hit
ACTCAAGAAGCAGAAAGGGAAGGTGAGGAGATAGAACAATGC19940.17428500755613785No Hit
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGC19400.16956515278781717No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG19090.16685560653192938No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT18480.16152391873808564No Hit
AAGCCCCAGTGATCTTCCAGTCTTTGTGTCAAGAAG17030.1488502346379653No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCA13080.1143253710548788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)