FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236061
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5368722.74285036494804No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA221309.37469552361466No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG188327.977599010425271No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC183677.780616027213306No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG174397.387497299426843No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA163656.932530151105011No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC156786.641503679133782No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG127495.400722694557762No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA120255.094022307793324No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT106604.515781937719487No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA95664.052342403022948No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC35871.5195224963039216No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCAGGTTTTGCCTCAG9410.3986257789300223No Hit
ACTCAAGAAGCAGAAAGGGAAGAGCACACGTCTGAACTCCAGTCACTCTC8150.34524974476936043TruSeq Adapter, Index 3 (96% over 31bp)
GCCATTCAAACCAGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGC7420.3143255345016754TruSeq Adapter, Index 8 (96% over 32bp)
ACAAACTGGTGGTGGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACG5590.23680319917309509No Hit
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGC5370.22748357416091602No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCCGT4070.17241306272531254TruSeq Adapter, Index 8 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)