FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48077465
Sequences flagged as poor quality0
Sequence length150
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21078654.384309780060159Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4119380.8568213819093831Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAAGCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2661040.5534900810598063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2296730.477714455202661Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1342440.27922437258287225Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1267520.2636411882365262Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1176100.24462604257524811Clontech Universal Primer Mix Long (96% over 26bp)
ACAAGCTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1166770.24268542444989558No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1132480.23555318484450044Clontech Universal Primer Mix Long (96% over 26bp)
ACAAGCTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1111980.23128923290776665No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1084890.22565457642161457Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1067420.2220208573808956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1023630.21291264004872135Clontech Universal Primer Mix Long (96% over 26bp)
ACAAGCTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1005370.20911460286019656No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT942390.19601491051992864Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG928480.19312166313261317Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT919410.19123512439767779Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC915940.1905133725332648Clontech Universal Primer Mix Long (96% over 25bp)
ACAAGCTATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900100.18721868967093003No Hit
ACAAGCTATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT888590.1848246366566956No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC853420.17750935911450405Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC800950.16659572213302012Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA795200.16539973561418014Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC792560.16485062180379104Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG762070.15850877328910748Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC760550.15819261685282282Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG757400.15753742423815398Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA747440.15546576758986771Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT733980.15266611914750497Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA697680.14511580425465442Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAAGCTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670350.13943122833119426No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT658930.13705589510595037Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG657730.13680629791940985Clontech Universal Primer Mix Long (96% over 26bp)
ACAAGCTATTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT655080.13625510413246622No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG646590.1344892040376921Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA616140.12815567542922657Clontech Universal Primer Mix Long (96% over 26bp)
ACAAGCTAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT606590.12616929781967498No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT593950.12354020745478156Clontech Universal Primer Mix Long (96% over 26bp)
ACAAGCTATTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT588480.12240246027946772No Hit
ACAAGCTATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT583500.1213666319553246No Hit
ACAAGCTATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579660.12056792095839497No Hit
ACAAGCTATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536020.11149090327453828No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT516610.10745366878224549Clontech Universal Primer Mix Long (96% over 26bp)
ACAAGCTATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507120.10547977103202093No Hit
ACAAGCTATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502970.10461658076190165No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501180.10424426495864539No Hit
ACAAGCTATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494430.10284028078435499No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA486920.10127821839192228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)