FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32128426
Sequences flagged as poor quality0
Sequence length150
%GC12

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6435842.0031606901626615Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2114500.6581399287970098Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1517110.47220178168703314No Hit
AAGGTACACGTCTGACCCATGTACTCTGCGTTGATACCACTGCTTCCCAT1494050.46502433701545165Clontech Universal Primer Mix Long (96% over 26bp)
AAGGTACATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1048390.32631228184038646No Hit
AAGGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1004410.3126234693227736Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1002200.3119356049375092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT997100.3103482255868993No Hit
AAGGTACAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT927110.2885637783811756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA905770.28192168517685867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT837720.2607410646260729No Hit
AAGGTACAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770600.23984990736863362No Hit
AAGGTACATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT668340.20802139513463871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA612520.19064737251678623Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC594890.18516001997732476Clontech Universal Primer Mix Long (96% over 26bp)
AAGGTACATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT594100.18491413180340674No Hit
AAGGTACATATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT584080.18179539825573776No Hit
AAGGTACATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT531160.1653240031117615No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA529830.16491003947719068No Hit
AAGGTACATTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524450.16323550988772373No Hit
AAGGTACATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510060.15875661011217915No Hit
AAGGTACATTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497440.15482862434655217No Hit
AAGGTACATTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491770.1530638320096976No Hit
AAGGTACATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481670.14992019839378373No Hit
AAGGTACAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477310.14856314467443876No Hit
AAGGTACATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469620.1461696256144014No Hit
AAGGTACATTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469090.14600466266227918No Hit
AAGGTACATTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461640.1436858438069764No Hit
AAGGTACATTTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454760.1415444379379183No Hit
AAGGTACATTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450220.14013135906502236No Hit
AAGGTACATTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433710.13499260748098896No Hit
AAGGTACACGTCTGACCCATGTACTCTGCGTTGATACCACTGCTTAGATC430580.13401839231090873Clontech SMART CDS Primer II A (96% over 26bp)
AAGGTACAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418460.13024603197181212No Hit
AAGGTACATTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413270.1286306400444267No Hit
AAGGTACATTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402720.12534694354463552No Hit
AAGGTACAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394660.12283826166896566No Hit
AAGGTACATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393810.12257369844386401No Hit
AAGGTACATATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389170.12112949448566201No Hit
AAGGTACAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382630.11909391390664455No Hit
AAGGTACATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376130.11707078336174949No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC372560.11595961781632252Clontech Universal Primer Mix Long (96% over 26bp)
AAGGTACATGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372450.11592538022248584No Hit
AAGGTACATAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370910.11544605390877226No Hit
AAGGTACATATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369280.11493871501828319No Hit
AAGGTACATGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366020.11392403723730504No Hit
AAGGTACATATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365300.11369993662310131No Hit
AAGGTACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363910.11326729793734683No Hit
AAGGTACATATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362860.11294048454163301No Hit
AAGGTACAAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358510.11158654333081863No Hit
AAGGTACATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339230.10558562688380688No Hit
AAGGTACATGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335550.10444022374454323No Hit
AAGGTACAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334910.10424102319858435No Hit
AAGGTACATTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334300.10405116017821725No Hit
AAGGTACATTTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331770.1032636955199735No Hit
AAGGTACAAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331410.10315164521287161No Hit
AAGGTACATATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330670.10292131958160665No Hit
AAGGTACATATTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330590.10289641951336179No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA330210.1027781441891987Clontech Universal Primer Mix Long (96% over 26bp)
AAGGTACATATTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329510.10256026859205615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)