FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33195327
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT12728573.834446336377406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCACTTTGCTCGGGTCACG2391760.7205110526550921Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC2059850.620524087622333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1765620.5318881178667105Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA1229910.3705069692490151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTATCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT741900.22349531306017864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACTTTGCTCGGGTCAC519970.15663951736339274Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTATCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509440.15346738412909744No Hit
ACGTATCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494620.14900290031786703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCACCTTTCTCGGGTCACG461360.1389834177563607Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA444620.13394053928132715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACTTTGCTCGGGTCACGGCC376120.11330510466126753Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG365020.10996126051115568Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTATCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361720.10896714468274404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)