FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31950480
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT7836012.4525484437166516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG4138041.295141731830007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC2010900.6293802158840807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1120750.3507772027212111Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462180.14465510377308888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATTTGAAGTAGATAGA428000.13395729892007882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCACAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427690.13386027377366475No Hit
CAACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA426950.13362866535964404Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC348310.10901557660479592Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)