FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32737056
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT9069602.77043849025398Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG5317891.624425238482043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3528401.0778000318660297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC2591900.7917327691286595Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1396120.42646473769663346Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA849500.25949187367367427Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT468860.14321996455637306Clontech Universal Primer Mix Long (96% over 26bp)
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT334360.10213502399238343Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC333270.10180206796848196Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)